Home > IX International Pear Symposium > PYRUS MICROSATELLITE MARKERS FROM GENBANK SEQUENCES

Articles

PYRUS MICROSATELLITE MARKERS FROM GENBANK SEQUENCES

Article number

671_41

Pages

289 – 292

Language

English

Abstract

Simple sequence repeat (SSR) or microsatellite markers have become the marker of choice for genotype identification.
A method was developed to identify microsatellite-containing SSRs from existing pear GenBank sequences.
We screened 366 genomic, mRNA and expressed sequence tag (EST) Pyrus nuclear sequences and identified 47 that contained SSRs.
Primer pairs were designed for 18 sequences and the optimum annealing temperature was determined by gradient PCR. The SSR primers were able to amplify a product in eight cultivars of P. communis, three cultivars of P. pyrifolia and one Pyrus hybrid (Nijisseiki × Anjou). Three primer pairs amplified fragments larger than the expected size and were not pursued further.
The remaining 15 primer pairs amplified fragments of the expected size, and 10 of them appeared to show polymorphism.
These 10 polymorphic loci are being used to characterize the Pyrus collection of the U.S. Department of Agriculture, Agricultural Research Service, National Clonal Germplasm Repository.

Publication

IX International Pear Symposium

Authors

N.V. Bassil, J.D. Postman, C. Neou

Keywords

pear, fingerprint

Full text

https://doi.org/10.17660/ActaHortic.2005.671.41

Online Articles (88)

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14. FLUORESCENCE IMAGING TO STUDY PHYSIOLOGICAL CHANGES IN ‘CONFERENCE’ PEARS DURING A SHELF-LIFE EXPERIMENT

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19. THE SYNTHETIC CYTOKININS CPPU AND TDZ PROLONG THE PHASE OF CELL DIVISION IN DEVELOPING PEAR (PYRUS COMMUNIS L.) FRUIT

20. STUDY ON COMPATIBILITY AND POLLEN TUBE GROWTH OF SOME ASIAN PEAR (PYRUS SEROTINA REHD.) CULTIVARS

K. Arzani | M. Koushesh-Saba | F. Ghanati

21. REPRODUCTIVE BUD DEVELOPMENT OF PEARS (PYRUS COMMUNIS L.) WITH EMPHASIS ON THE BOURSE SHOOT

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22. THE EFFECT OF SCORING DURING FLOWER INDUCTION OR THE INITIATION PHASE ON RETURN BLOOM IN PYRUS COMMUNIS L.

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23. FLOWERING DATE PREDICTION IN ‘ROCHA’ PEAR UNDER MILD WINTER CONDITIONS

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24. PROGRESS AND CHALLENGES OF THE SOUTH AFRICAN PEAR BREEDING PROGRAM

J.P. Human

25. PROGRESS IN PEAR BREEDING AT THE UNIVERSITY OF BOLOGNA

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26. CHARACTERISTICS OF AN F1 GENERATION ARISING FROM BUTIRRA PRECOCE MORETTINI x BELLA DI GIUGNO

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27. ORCHARD COMPARISONS OF NEW QUINCE AND PYRUS ROOTSTOCK CLONES

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28. PROGRESS IN THE NATIONAL ASIAN PEAR PROJECT: A STUDY ON THE ADAPTATION OF SOME ASIAN PEAR (PYRUS SEROTINA REHD) CULTIVARS TO IRANIAN ENVIRONMENTAL CONDITIONS

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30. ‘ROCHA’, THE PEAR FROM PORTUGAL

J.M. Silva | N.G. Barba | M.T. Barros | A. Torres-Paulo

31. HERITABILITY OF MORPHOLOGICAL AND ARCHITECTURAL CHARACTERS IN THREE PEAR PROGENIES

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32. BREEDING PEARS FOR WARM CLIMATES IN MEXICO

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36. VARIABILITY IN THE WILD PEAR POPULATION IN WEST SERBIA

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37. A SELECTION FROM PYRUS BETULAEFOLIA AS A NEW POLLINATOR FOR THE MAIN PYRUS COMMUNIS CULTIVARS

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61. AN EVALUATION OF SITE-SPECIFIC MANAGEMENT STRATEGIES IN THE U.S. PEAR INDUSTRY

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F.M. Maas

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M.F. Addison

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R.B. Elkins | K.M. Klonsky | R.L. DeMoura

80. MONITORING CODLING MOTH IN FOUR PEAR CULTIVARS WITH THE PEAR ESTER

A.L. Knight | P. VanBuskirk | R.J. Hilton | B.G. Zoller | D.M. Light

81. RESISTANCE OF SOME PYRUS COMMUNIS CULTIVARS AND PYRUS HYBRIDS TO THE PEAR PSYLLA CACOPSYLLA PYRI (HOMOPTERA, PSYLLIDAE)

P. Robert | T. Raimbault

82. THE CHALLENGES OF IMPLEMENTING A SUCCESSFUL IPM PROGRAM IN PEAR

P.D. VanBuskirk | R.J. Hilton

83. CONTROL OF FIRE BLIGHT BY PSEUDOMONAS FLUORESCENS A506 INTRODUCED INTO UNOPENED PEAR FLOWERS

R.B. Elkins | C.A. Ingels | S.E. Lindow

84. FRUIT AND LEAF INCIDENCE OF PEAR SCAB (VENTURIA PIRINA ADERH.) IN MIXED EUROPEAN AND ASIAN PEAR PROGENIES

L. Brewer | P. Alspach | V. Bus

85. SCAB RESISTANCE IN PYRUS GERMPLASM

J.D. Postman | R.A. Spotts | J. Calabro

86. POWDERY MILDEW RESISTANCE IN PYRUS GERMPLASM

M. Serdani | R.A. Spotts | J.M. Calabro | J.D. Postman

87. TEST OF FUNGICIDES AGAINST STEMPHYLIUM VESICARIUM ON PEAR WITH OR WITHOUT A WARNING SYSTEM

P.F. de Jong | B. Heijne | A. Boshuizen

88. AGE DETERMINATION AND TREE-RING GROWTH DYNAMICS IN OLD TREES OF PYRUS COMMUNIS ‘ANGELICA’

D. Neri | C. Urbinati | G. Savini | A. Sanchioni