Home > IX International Pear Symposium > DEVELOPMENT OF MICROSATELLITE MARKERS FOR MARKER-ASSISTED BREEDING IN PEARS (PYRUS SPP.)

Articles

DEVELOPMENT OF MICROSATELLITE MARKERS FOR MARKER-ASSISTED BREEDING IN PEARS (PYRUS SPP.)

Article number

671_44

Pages

307 – 313

Language

English

Abstract

With the construction of a reference linkage map for apple (Malus spp.), consisting primarily of microsatellite markers, it is now possible to exploit this information to develop genetic maps for pear (Pyrus spp.) also, and thus develop markers for use in marker assisted breeding programs.
A cross-species survey has shown that it is possible to amplify microsatellite repeats (SSRs) using Malus primers on Pyrus. We are currently implementing SSRs obtained through literature for Malus and Pyrus. In addition, we are developing new SSR markers from the analysis of genomic and cDNA sequences from Malus and Pyrus. Candidate SSRs have been identified with Tandem Repeats Finder, and then optimized for PCR and tested for polymorphism in both apple and pear.
Markers are then multiplexed together for higher throughput screening and mapping.

Publication

IX International Pear Symposium

Authors

M.M. van Dyk, G. Koning, Z. Simayi, S. Booi, R. Maharaj, M.C. Selala, D.J.G. Rees

Keywords

PCR, cross-species survey, simple sequence repeats, Malus spp.

Full text

https://doi.org/10.17660/ActaHortic.2005.671.44

Online Articles (88)

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7. STATUS OF THE PEAR INDUSTRY IN ASIA

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9. STATUS OF THE PEAR INDUSTRY IN AFRICA, WITH SPECIFIC REFERENCE TO SOUTH AFRICA

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14. FLUORESCENCE IMAGING TO STUDY PHYSIOLOGICAL CHANGES IN ‘CONFERENCE’ PEARS DURING A SHELF-LIFE EXPERIMENT

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17. APPLICATION OF S-RNASE ALLELE MOLECULAR ANALYSIS AS A MEANS FOR ELUCIDATING LOW YIELDS IN THE PEAR ORCHARD

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21. REPRODUCTIVE BUD DEVELOPMENT OF PEARS (PYRUS COMMUNIS L.) WITH EMPHASIS ON THE BOURSE SHOOT

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22. THE EFFECT OF SCORING DURING FLOWER INDUCTION OR THE INITIATION PHASE ON RETURN BLOOM IN PYRUS COMMUNIS L.

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23. FLOWERING DATE PREDICTION IN ‘ROCHA’ PEAR UNDER MILD WINTER CONDITIONS

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24. PROGRESS AND CHALLENGES OF THE SOUTH AFRICAN PEAR BREEDING PROGRAM

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25. PROGRESS IN PEAR BREEDING AT THE UNIVERSITY OF BOLOGNA

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26. CHARACTERISTICS OF AN F1 GENERATION ARISING FROM BUTIRRA PRECOCE MORETTINI x BELLA DI GIUGNO

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27. ORCHARD COMPARISONS OF NEW QUINCE AND PYRUS ROOTSTOCK CLONES

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30. ‘ROCHA’, THE PEAR FROM PORTUGAL

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M.F. Addison

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R.B. Elkins | K.M. Klonsky | R.L. DeMoura

80. MONITORING CODLING MOTH IN FOUR PEAR CULTIVARS WITH THE PEAR ESTER

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P.D. VanBuskirk | R.J. Hilton

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R.B. Elkins | C.A. Ingels | S.E. Lindow

84. FRUIT AND LEAF INCIDENCE OF PEAR SCAB (VENTURIA PIRINA ADERH.) IN MIXED EUROPEAN AND ASIAN PEAR PROGENIES

L. Brewer | P. Alspach | V. Bus

85. SCAB RESISTANCE IN PYRUS GERMPLASM

J.D. Postman | R.A. Spotts | J. Calabro

86. POWDERY MILDEW RESISTANCE IN PYRUS GERMPLASM

M. Serdani | R.A. Spotts | J.M. Calabro | J.D. Postman

87. TEST OF FUNGICIDES AGAINST STEMPHYLIUM VESICARIUM ON PEAR WITH OR WITHOUT A WARNING SYSTEM

P.F. de Jong | B. Heijne | A. Boshuizen

88. AGE DETERMINATION AND TREE-RING GROWTH DYNAMICS IN OLD TREES OF PYRUS COMMUNIS ‘ANGELICA’

D. Neri | C. Urbinati | G. Savini | A. Sanchioni