DETERMINATION OF BIOACTIVE COMPOUNDS IN ROOTS OF DIFFERENT AGES PUERARIA MIRIFICA, AIRY SHAW SUVATABHANDHU AND BUTEA SUPERBA, ROXB. FROM VARIOUS LOCATIONS IN THAILAND

Bioactive compounds from roots of White Kwao Krua (Pueraria mirifica) and Red Kwao Krua (Butea superba) extracted by organic solvents were determined from HPLC fingerprints and compared with standard isoflavonoids (puerarin, daidzein and genistein). Roots of White Kwao Krua from Chiang Mai province harvested at the age of 6 y old gave the highest amounts of puerarin, daidzein and genistein at 290, 89 and 16 mg/kg of the dried root respectively.
Roots that were younger or older than 6 y old appeared to contain less amount of active compounds.
Red Kwao Krua from Chiang Mai had the highest amount of the active compounds.
Puerarin contents in Red Kwao Krua were lower whereas daidzein and genistein were greater than those found in White Kwao Krua.
The contents of puerarin, daidzein and genistein in Red Kwao Krua from Chiang Mai were 1.9, 37.2 and 4.5 mg/kg of the dried root respectively.
For miroestrol contents, the highest amount of 45.0 mg/kg of the dried root was found in White Kwao Krua collected from Chiang Mai province at the age of 5.5 y old.
No miroestrol could be determined from roots of Red Kwao Krua.
This study suggested that both isoflavonoids and miroestrol contents in the two plants depended on ages and locations of cultivation in Thailand.
Information from this study can be applied for the selection of sources and ages of the plants that contain high amounts of the bioactive compounds for herbal formulations.

III WOCMAP Congress on Medicinal and Aromatic Plants – Volume 6: Traditional Medicine and Nutraceuticals

A. Manosroi, J. Manosroi

daidzein, finger prints, genistein, HPLC, miroestrol, puerarin, sites

https://doi.org/10.17660/ActaHortic.2005.680.12