Home > III International Chestnut Congress > REINVIGORATION OF MATURE CASTANEA SATIVA BY SERIAL MICROGRAFTING ONTO A JUVENILE CLONE

Articles

REINVIGORATION OF MATURE CASTANEA SATIVA BY SERIAL MICROGRAFTING ONTO A JUVENILE CLONE

Article number

693_36

Pages

293 – 298

Language

English

Abstract

In vitro micrografting of mature chestnut (Castanea sativa Mill.) onto a juvenile clone was carried out in order to estimate wether in vitro reinvigoration takes place.
In vitro cultured shoot tips of two selected mature clones were micrografted onto in vitro rooted shoots of juvenile origin using the slit grafting method.
When the growing scion reached about 4 cm it was cut in two segments which were subcultured to obtain a stock for evaluation.
By repeating this procedure, stocks of one-, two- and three-times micrografted microshoots were obtained.
Multiplication and rooting rates of micrografted material were evaluated and compared to those of control and the juvenile clone used as rootstock.
The graft-take was in the range 64-78%. Multiplication rate of 1-, 2- and 3-times micrografted material was higher than that of ungrafted one all along 8-12 subcultures for one of the adult clones.
There were no significant differences between multiplication rates of 1-, 2- and 3-times micrografted shoots.
On the other hand, micrografting did not improve in vitro rooting.

Publication

III International Chestnut Congress

Authors

J.L. Fernández-Lorenzo, M.J. Fernández-López

Keywords

chestnut, micropropagation, slit grafting

Full text

https://doi.org/10.17660/ActaHortic.2005.693.36

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