Home > III International Chestnut Congress > VARIETAL IDENTIFICATION OF CHESTNUT USING MICROSATELLITES MARKERS

Articles

VARIETAL IDENTIFICATION OF CHESTNUT USING MICROSATELLITES MARKERS

Article number

693_55

Pages

441 – 446

Language

English

Abstract

Microsatellites (SSRs) combine several features of an ultimate molecular marker and they are used increasingly in many plant genetic studies and applications.
In this work, we report on the utilisation of six SSRs markers, previously developed in chestnut (Castanea sativa Mill.), for the identification and differentiation of a set of forty varietal denominations.
DNA was isolated from lyophilised leaves and PCR products were separated on an ABI PRISM 310 analyser.
All studied microsatellite markers revealed a high level of polymorphism that allowed unique genotyping of most of the examined denominations, as well as identification of very probable synonymies and homonymies.
Data obtained suggest that the varietal panorama in Andalusia is extremely complex, the number of the genotypes being higher than the one indicated by the knowledge of the farmers and other previous researches.

Publication

III International Chestnut Congress

Authors

A.C. Martin, M.J. Giménez, J.B. Alvarzez

Keywords

chestnut, SSRs, molecular markers, varietal identification

Full text

https://doi.org/10.17660/ActaHortic.2005.693.55

Online Articles (99)

1. THE CHESTNUT: A MULTIPURPOSE RESOURCE FOR THE NEW MILLENNIUM

G. Bounous

2. CHESTNUTS IN THE UNITED STATES FOR FOOD AND FOR TIMBER

S.L. Anagnostakis

3. CHESTNUT RESEARCH IN CROATIA

S. Novak Agbaba | B. Liovic | J. Medak | D. Slade

4. THE COMPETITIVE ADVANTAGE OF CHILEAN NATIONAL FRESH CHESTNUT INDUSTRY

J.P. Joublan | D. Rios | J.C. Montigaud

5. STABILITY AND QUALITY OF TRADITIONAL AND INNOVATIVE CHESTNUT BASED PRODUCTS

G. Sacchetti | P. Pittia | D. Mastrocola | G.G. Pinnavaia

6. ´MARRON GLACÉ´ QUALITY AFFECTED BY STORAGE CONDITIONS OF THE NUT

A.M. Estévez | L.A. Lizana | M.X. González

7. BASIC TREE ARCHITECTURAL TRAITS OF DIFFERENT CHESTNUT CULTIVARS RESEARCH IN SLOVENIA

A. Solar | F. Stampar

8. THE CHESTNUT AND ITS RESOURCES: IMAGES AND CONSIDERATIONS

E. Bellini

9. MARRONE DEL MUGELLO PGI CHESTNUT NUTRITIONAL AND ORGANOLEPTIC QUALITY

E. Bellini | E. Giordani | C. Marinelli | B. Perucca

10. TEMPERATURE EFFECT ON SUGAR CONCENTRATION AND MOISTURE PROFILES DURING OSMOTIC DEHYDRATION OF CHESTNUT FRUITS

F. Chenlo | R. Moreira | C. Fernández-Herrero

11. DRYING KINETICS OF CHESTNUT (CASTANEA SATIVA MILLER): DEPENDENCY OF THE VARIETY AND EXPERIMENTAL CONDITIONS

F. Chenlo | R. Moreira | L. Chaguri | F. Santos

12. RESEARCH ACTIVITIES ON CHESTNUT: RECOMMENDATIONS FOR TERMINOLOGY AND MEASUREMENT STANDARDS

13. REHYDRATION OF DRIED CHESTNUTS: INFLUENCE OF VARIETY AND PROCESS CONDITIONS

G. Sacchetti | D. Mastrocola | G.G. Pinnavaia

14. THE TRANSFERRING OF TRADITIONAL CULTIVAR´S CHESTNUT SCIONS TO COMMERCIAL NURSERIES

G. Bustingorri | P. Furones | J. Fernández-López

15. CHINESE CHESTNUT CULTIVAR PERFORMANCE IN MISSOURI

K.L. Hunt | M.A. Gold | M.R. Warmund

16. REMOVAL OF SECONDARY BURS INCREASES AVERAGE NUT WEIGHT FROM PRIMARY BURS OF ´ARMSTRONG´, ´ORRIN´ AND ´WILLAMETTE´ CHINESE CHESTNUTS

M.R. Warmund | K.L. Hunt | M.A. Gold

17. THE INFLUENCE OF DRYING ON THE CHEMICAL PROPERTIES OF THE CHESTNUTS

R.M.C. Fernandes | R.P.F. Guiné | P.M.R. Correia

18. DISTRIBUTION AND HEALTH CONDITION OF SWEET CHESTNUT (CASTANEA SATIVA MILL.) IN THE CZECH REPUBLIC

P. Haltofová | D. Palovciková | L. Jankovský

19. INVESTIGATION OF ANATOMICAL STRUCTURE OF GRAFT UNION FOR T AND INVERTED T BUDDINGS AND WHIP GRAFTING IN CHESTNUT

U. Serdar | A. Soyla

20. THE INVENTORY OF THE GIANT CHESTNUT TREES IN SOUTHERN SWITZERLAND

P. Krebs | M. Conedera | P. Fonti

21. GEOGRAPHIC VARIABILITY AMONG EXTREME EUROPEAN WILD CHESTNUT POPULATIONS

22. THE EFFECT OF GRAFTING TIME AND METHODS ON CHESTNUT NURSERY TREE PRODUCTION

U. Serdar | A. Soylu

23. PHENOLIC COUMPOUNDS AS A FACTOR OF DIFFERENT ROOTING RESPONSE IN DIFFERENT CHESTNUT HYBRID CLONES (CASTANEA CRENATA X CASTANEA SATIVA)

G. Osterc | M. Trobec | A. Solar | F. Stampar

24. CARBON SEQUESTRATION IN A SPANISH CHESTNUT COPPICE

J.F. Gallardo Lancho | M.I. Gonzalez

25. MYCORRHIZAL INOCULATION OF CASTANEA SATIVA MILL. MICROPROPAGATED PLANTS: EFFECT OF MYCORRHIZATION ON GROWTH

A. Martins | M.S. Pais

26. POST CULTURAL DYNAMICS IN A MIXED CHESTNUT COPPICE AT ITS ECOLOGICAL BORDER

M. Pividori | F. Armando | M. Conedera

27. CRYOPRESERVATION OF EUROPEAN CHESTNUT GERMPLASM

M.C. San-José | L. Jorquera | N. Vidal | E. Corredoira | C. Sánchez

28. PHENOTYPIC VARIATION AND POPULATION RELATIONSHIPS OF CHESTNUT (CASTANEA SATIVA) IN GREECE, REVEALED BY MULTIVARIATE ANALYSIS OF LEAF MORPHOMETRICS

F.A. Aravanopoulos

29. COMPOSITIONAL CHARACTERISTICS OF SOME CHESTNUT BIOTYPES OF EMILIANO-ROMAGNOLO APENNINE

G. Sacchetti | G.G. Pinnavaia

30. GENETIC VARIATION IN DROUGHT RESPONSE OF CASTANEA SATIVA MILL. SEEDLINGS

G. Eriksson | A. Jonsson | M. Lauteri | A. Pliura

31. LEAF NITROGEN AS A FACTOR OF SUSTAINABLE DEVELOPMENT IN TWO CHESTNUT FOREST ECOSYSTEMS IN SALAMANCA AREA, SPAIN

I. Santa Regina | S. Salazar

32. PRELIMINARY DIVERSITY STUDIES OF CULTURABLE PHYLLOSPHERE BACTERIA ON CHESTNUT (CASTANEA SATIVA)

A. Valverde | J.M. Igual | I. Santa Regina | S. Salazar | C. Rodriguez-Barrueco

33. EFFECT OF YEAR AND CULTIVAR ON CHEMICAL COMPOSITION OF CHESTNUTS FROM NORTHEASTERN PORTUGAL

J.V. Ferreira-Cardoso | J.M.G. Torres-Pereira | C.A. Sequeira

34. ACCLIMATION OF CHLOROPLASTS FROM NORTH AND SOUTH EXPOSED CANOPY SECTORS OF CHESTNUT TREES (CASTANEA SATIVA MILL.)

J. Gomes-Laranjo | J.P. Coutinho | F. Peixoto | J. Torres-Pereira

35. IDENTIFICATION AND MORPHOLOGICAL DESCRIPTION OF CHESTNUT CULTIVARS OF THE REGION OF VERÍN-MONTERREI (OURENSE, SPAIN)

J.M. Queijeiro | D. Blanco | J. De la Montaña | M. Miguez

36. REINVIGORATION OF MATURE CASTANEA SATIVA BY SERIAL MICROGRAFTING ONTO A JUVENILE CLONE

J.L. Fernández-Lorenzo | M.J. Fernández-López

37. COMPARISON OF NUT CHARACTERISTICS BETWEEN KOREAN NATIVE CHESTNUT ACCESSIONS AND PREVAILING CULTIVARS CULTIVATED IN KOREA

M.J. Kim | U. Lee | S.C. Kim | M.S. Hwang | M.H. Lee

38. COST AND EFFICIENCY OF PROPAGATING CASTANEA SATIVA HYBRIDS IN VITRO AND BY CUTTING IN A COMMERCIAL NURSERY

L. Rodriguez | B. Cuenca | B. Pato | M.J. Cámara | L. Ocaña

39. EFFECT OF GENOTYPE ON MICROPROPAGATION AND POST-PROPAGATION GROWTH OF 35 COMMERCIAL CLONES OF CASTANEA SP.

M.E. Miranda-Fontaiña | J. Fernández-López

40. EVALUATION OF INRA CHESTNUT INTERSPECIFIC HYBRIDS

M. Ramos Guedes-Lafargue | R. Franzini | F. Laigret

41. TECHNOLOGICAL AND SENSORY CHARACTERISTICS OF CHESTNUT (CASTANEA SATIVA MILL.) FRUITS FROM DIFFERENT SEEDLING ORCHARDS IN SLOVAKIA

M. Bolvansky | J. Brindza | V. Horcin

42. IN VITRO PROLIFERATION AND GENOME DNA METHYLATION IN ADULT CHESTNUTS

43. NUTRIENT POOLS TO THE SOIL THROUGH ORGANIC MATTER IN SEVERAL CASTANEA SATIVA MILL. COPPICES OF MOUNTAINOUS MEDITERRANEAN CLIMATE AREAS

I. Santa Regina | S. Salazar | S. Leonardi | M. Rapp

44. ENDOGENOUS CONTAMINATION IN MICROPROPAGATION OF CASTANEA SATIVA MILL.

S. Ferrador | G. Marques | H. Guedes-Pinto

45. ECOLOGICAL CHARACTERISTICS, DISTRIBUTION AND MANAGEMENT OF CASTANEA SATIVA MILL. ECOSYSTEMS IN BULGARIA

S. Bratanova-Dancheva | V. Dimitrova | M. Lyubenova | S. Mihaylov

46. DENDROCHRONOLOGICAL INVESTIGATION OF MISTLETOE GROWTH IMPACT (LORANTHUS EUROPAEUS L.) ON EUROPEAN CHESTNUT (CASTANEA SATIVA MILL.) IN SOUTH WEST BULGARIA

S. Bratanova-Dancheva | S. Mirtchev | M. Lyubenova

47. MANAGEMENT OF GENETIC RESOURCES OF THE MULTI-PURPOSE TREE SPECIES CASTANEA SATIVA MILL.

48. GENETIC TRANSFORMATION OF CASTANEA SATIVA MILL. BY AGROBACTERIUM TUMEFACIENS

E. Corredoira | M.C. San-José | A. Ballester | A.M. Vieitez

49. GENETIC CHARACTERIZATION AND NUT QUALITY OF CHESTNUT CULTIVARS FROM PIEMONTE (ITALY)

R. Botta | A. Akkak | P. Guaraldo | G. Bounous

50. MOLECULAR POPULATION GENETICS AND DYNAMICS OF CHESTNUT (CASTANEA SATIVA) IN EUROPE: INFERENCES FOR GENE CONSERVATION AND TREE IMPROVEMENT

51. GENETIC VARIATION OF FRUIT TRAITS IN HELLENIC CHESTNUT (CASTANEA SATIVA MILL.) POPULATIONS: A FIRST ASSESSMENT

P.G. Alizoti | F.A. Aravanopoulos

52. THE DIVERSITY OF CASTANEA RESOURCES AND CULTIVARS IMPROVEMENT IN CHINA

L. Qin | Y.Q. Feng | H.M. Xu | Q.H. Dong | X.H. Gao

53. ANALYSIS OF GENETIC VARIATION IN SPANISH CHESTNUT POPULATIONS FOR SELECTING SEED STANDS

R. Blanco Silva | J. Fernández-López

54. CHARACTERISATION OF TRADITIONAL PORTUGUESE CHESTNUT CULTIVARS BY NUCLEAR SSRS

55. VARIETAL IDENTIFICATION OF CHESTNUT USING MICROSATELLITES MARKERS

A.C. Martin | M.J. Giménez | J.B. Alvarzez

56. MULTILOCUS GENETIC STRUCTURE OF EUROPEAN CHESTNUT (CASTANEA SATIVA) HELLENIC CLONES AND GENETIC DIVERSITY OF ORCHARD POPULATIONS

F.A. Aravanopoulos | A.D. Drouzas

57. APPLICATION AND EVALUATION OF MORPHOLOGICAL, PHENOLOGICAL AND MOLECULAR TECHNIQUES FOR THE CHARACTERIZATION OF CASTANEA SATIVA MILL. CULTIVARS

G.L. Beccaro | R. Botta | D. Torello Marinoni | A. Akkak | G. Bounous

58. COTYLEDON STORAGE PROTEINS IN EUROPEAN SWEET CHESTNUT

M.A. Martin | L.M. Martin | J.B. Alvarez

59. CHESTNUT CULTIVAR EVALUATIONS IN TENNESSEE: ORCHARD ESTABLISHMENT AND EARLY GERMPLASM CHARACTERIZATION

J.H. Craddock | M.T. Alexander | S.H. Alexander | J.L. Bramblett | L.M. Worthen

60. GENETIC VARIABILITY OF CHESTNUT IN ANDALUSIA (SPAIN)

J.B. Alvarez | M.A. Martin | C. Muñoz | S. López | L.M. Martin

61. THE MODE OF INHERITANCE OF WEEPING HABIT IN JAPANESE CHESTNUT, CASTANEA CRENATA

K. Kotobuki | Y. Sawamura | T. Saito | N. Takada

62. CONSERVATION OF CASTANEA DENTATA GERMPLASM OF THE SOUTHEASTERN UNITED STATES

M.T. Alexander | L.M. Worthen | J.H. Craddock

63. AN IMPROVED GENETIC MAP FOR CASTANEA MOLLISSIMA/CASTANEA DENTATA AND ITS RELATIONSHIP TO THE GENETIC MAP OF CASTANEA SATIVA

64. DIFFERENTIATION AMONG CHESTNUT CULTIVARS USING ADAPTATIVE AND MORPHOLOGICAL TRAITS

P. Furones | J. Fernández-López

65. DNA-TYPING OF CULTIVARS FOR THE DEVELOPMENT OF A CHESTNUT INDUSTRY IN CHILE

66. OPTIMIZING CONDITIONS FOR SUCCESSFUL PLANT REGENERATION FROM CRYOPRESERVED CASTANEA SATIVA SHOOT TIPS

L. Jorquera | N. Vidal | C. Sánchez | A.M. Vieitez

67. PHYTOSANITARY CRITERIA FOR THE PROTECTION OF CHESTNUT ORCHARDS AND STANDS AGAINST CHESTNUT BLIGHT AND INK DISEASE

T. Turchetti | G. Maresi

68. MONITORING INK DISEASE OF CHESTNUT WITH THE AIRBORNE MULTISPECTRAL SYSTEM A.S.P.I.S.

69. CHARACTERIZATION OF CRYPHONECTRIA PARASITICA POPULATIONS IN SOUTHERN ITALY

A.M. Pennisi | G. Sammarco | D. Spica | S.O. Cacciola

70. ASSOCIATION OF THE BLACK ROT FUNGUS CIBORIA BATSCHIANA WITH THE CHESTNUT WEEVIL CURCULIO PROPINQUUS IN CHESTNUT ORCHARDS IN CENTRAL ITALY

A.M. Vettraino | S. Speranza | B. Paparatti | C. Pucci | A. Vannini

71. A NEW TRANSMISSIBLE SYMPTOMOLOGY ON SWEET CHESTNUT IN ITALY

72. MANAGEMENT OF CHESTNUT WEEVIL (CURCULIO SPP.)¹ , INSECT KEY-PEST IN CENTRAL ITALY

B. Paparatti | S. Speranza

73. NUTRIENT STATUS OF CHESTNUT ORCHARDS. I. RELATIONSHIP WITH INCIDENCE OF BLIGHT

E. Portela | J. Louzada

74. NUTRIENT STATUS OF CHESTNUT ORCHARDS. II. SEASONAL NUTRIENT VARIATION IN THE INNER BARK IN TWO ORCHARDS WITH DIFFERENT INCIDENCE OF BLIGHT

E. Portela | J. Louzada

75. SITE FACTORS AND MANAGEMENT PRACTICES ASSOCIATED WITH THE INCIDENCE OF BLIGHT IN CHESTNUT ORCHARDS IN NORTHEASTERN PORTUGAL

E. Portela | R. Pinto

76. SPREAD OF CHESTNUT BLIGHT IN TRADITIONAL AGRO-FORESTRY ECOSYTEMS OF TRÁS-OS-MONTES (PORTUGAL)

M.E. Gouveia | V. Coelho

77. DIAGNOSIS OF INK DISEASE OF CHESTNUT BY MOLECULAR IDENTIFICATION OF ASSOCIATED PHYTOPHTHORA SPECIES

78. EXAMINATION OF CRYPHONECTRIA PARASITICA (MURR.) BARR FUNGUS ON CHESTNUT IN THE EASTERN REGIONS OF THE CARPATHIAN BASIN

G. Tarcali | L. Radócz

79. OCCURRENCE AND DIVERSITY OF VEGETATIVE COMPATIBILITY TYPES OF CRYPHONECTRIA PARASITICA IN GALICIA (NW SPAIN)

O. Aguin | M. Mata | J.P. Mansilla | A. Romero

80. PHEROMONE TRIALS FOR THE MONITORING OF CYDIA SPLENDANA IN CHESTNUT PLANTATIONS OF FRANCE

81. CHARACTERISTICS OF HYPOVIRULENT STRAINS OF CHESTNUT BLIGHT FUNGUS, CRYPHONECTRIA PARASITICA ISOLATED IN KOREA

S.H. Lee | J.Y. Park | K.H. Kim | J.K. Lee

82. IDENTIFICATION OF NATURAL INFECTION OF QUERCUS SPP. BY CHESTNUT BLIGHT FUNGUS (CRYPHONECTRIA PARASISTICA)

L. Radócz | G. Tarcali

83. ASSESSMENT OF CHESTNUT INK DISEASE SPREAD BY GEOSTATISTICAL METHODS

L.M. Martins | F.W. Macedo | C.P. Marques | C.G. Abreu

84. CHESTNUT BLIGHT IN PORTUGAL – MONITORING AND VC TYPES OF CRYPHONECTRIA PARASITICA

85. DIVERSITY OF CRYPHONECTRIA PARASITICA (MURR.) BARR VEGETATIVE COMPATIBILITY (VC) TYPES IN SLOVAKIA

G. Juhásová | M. Kobza | K. Adamciková

86. SENSITIVITY TO COPPER AND PHOSPHITE OF PHYTOPHTHORA SPECIES ASSOCIATED WITH INK DISEASES OF CHESTNUT

V. Coelho | S. Coutinho | M.E. Gouveia

87. SELECTION OF CASTANEA SATIVA MILL. GENOTYPES RESISTANT TO INK DISEASE IN GALICIA (SPAIN)

L. Rodriguez | B. Cuenca | C.A. López | F.J. Lario | L. Ocaña

88. SOIL MICROBIOTA AND BIOCHEMICAL CHARACTERISTICS IN CHESTNUT STANDS OF THE SIERRA DE FRANCIA (SPAIN), UNDER DIFFERENT CONDITIONS OF EXPLOITATION AND MANAGEMENT

89. THE IMPORTANCE OF DEEP SOIL LAYERS TO SUPPLY WATER TO AGRO-FORESTRY SYSTEMS: A CASE STUDY OF A MATURE CHESTNUT ORCHARD IN NORTHERN PORTUGAL

90. EFFECT OF FERTILIZING AND PRUNING ON CHESTNUT LITTER PRODUCTION AND NUTRIENT RELEASE

A.L. Pires | A. Oliveira | F. João | C. Ribeiro

91. IMPACT OF MANAGEMENT PRACTICES ON CHESTNUT GROVE NUTRIENT BUDGETS

A.L. Pires | E. Portela

92. EFFECT OF SOIL TILLAGE ON DIVERSITY AND ABUNDANCE OF MACROFUNGI ASSOCIATED WITH CHESTNUT TREE IN THE NORTHEAST OF PORTUGAL

P. Baptista | A. Martins | T. Lino-Neto | R.M. Tavares

93. ORGANIC MATTER AND NUTRIENT DYNAMICS OF THE LITTER LAYER ON A TWO CHESTNUT STANDS IN HONFRIA AREA, SPAIN

S. Salazar | I. Santa Regina

94. EFFECT OF IRRIGATION AND SOIL MANAGEMENT PRACTICES ON FRUIT PRODUCTION AND QUALITY IN CHESTNUT ORCHARDS OF NORTHERN PORTUGAL

I. Linhares | A. Martins | O. Borges | C. Guedes | V. Sousa

95. ASSESSMENT OF A NEW CONCEPT OF CHESTNUT ORCHARD MANAGEMENT IN THE VEGETATIVE WALL

J. Gomes-Laranjo | J.P. Coutinho | J. Torres-Pereira | C. Ramos

96. CHESTNUT HIGH FOREST STANDS: BIOMETRICAL STUDY IN THE NORTH OF PORTUGAL

L.F. Nunes | M.L. Monteiro | M.S. Patricio | L.S. Martins | T. Alpuim

97. MANAGEMENT MODELS EVALUATION OF A CASTANEA SATIVA COPPICE IN THE NORTHEAST OF PORTUGAL

98. BIOMASS EQUATIONS FOR CASTANEA SATIVA HIGH FOREST IN THE NORTHWEST OF PORTUGAL

M.S. Patricio | M.L. Monteiro | M. Tomé

99. REDUCING THE RISK OF RING SHAKE IN CHESTNUT

P. Fonti | F. Giudici