Home > X International Workshop on Fire Blight > MECHANISMS INVOLVED IN FIRE BLIGHT PROTECTION OF APPLE WITH AN HRPL MUTANT OF ERWINIA AMYLOVORA

Articles

MECHANISMS INVOLVED IN FIRE BLIGHT PROTECTION OF APPLE WITH AN HRPL MUTANT OF ERWINIA AMYLOVORA

Article number

704_76

Pages

481 – 482

Language

English

Abstract

hrpL and hrpS mutants of Erwinia amylovora have been shown to be significantly more effective than hrp secretory mutants in preventing apple seedlings and apple flowers from developping fire blight symptoms caused by a virulent strain.
Mechanisms involved in the protective effect obtained with an hrpL mutant were investigated.
Two histochemical techniques (a two-step staining and an immunostaining technique) indicated that when simultaneously inoculated, cells of the two strains localized mainly in separate areas of the leaf tissues.
In addition, leaves infiltrated with the hrpL mutant exibited a significant increase in peroxidase activity in contrast to an hrp secretion mutant.
Mechanisms of the protective effect obtained with the hrpL mutant are proposed.

Publication

X International Workshop on Fire Blight

Authors

M. Faize, M.N. Brisset, J.P. Paulin, M. Tharaud, C. Perino, B. Vian, M.A. Barny

Keywords

histochemical assays, peroxidase, apple defense response

Full text

https://doi.org/10.17660/ActaHortic.2006.704.76

Online Articles (93)

1. PRESENT WORLDWIDE DISTRIBUTION OF FIRE BLIGHT AND CLOSELY RELATED DISEASES

T. van der Zwet

2. FIRST OUTBREAKS OF FIRE BLIGHT IN SLOVENIA

T. Dreo | M. Zupančič | T. Demsar | M. Ravnikar

3. TEN YEARS OF FIRE BLIGHT IN AUSTRIA: SURVEY AND CONTROL MEASURES

4. SEVERE OUTBREAK OF FIRE BLIGHT ON QUINCE IN TURKEY

H. Saygili | Y. Aysan | M. Mirik | F. Sahin

5. COSTS ASSOCIATED WITH FIRE BLIGHT INCURSION MANAGEMENT AND PREDICTED COSTS OF FUTURE INCURSIONS

B.C. Rodoni | P.R. Merriman | S.J. McKirdy | G. Wittwer

6. IMMUNOLIPOSOMES FOR EARLY DETECTION OF ERWINIA AMYLOVORA

B.S. Borejsza-Wysocki | R.A. Durst | A.S. Aldwinckle

7. FLUORESCENCE IMAGING OF THE INFECTION PATTERN OF APPLE LEAVES WITH ERWINIA AMYLOVORA

K. Heyens | R. Valcke

8. STABILITY OF SHORT SEQUENCE REPEATS AND SUITABILITY OF SSR, AFLP AND RAPD FOR THE CHARACTERIZATION OF ERWINIA AMYLOVORA STRAINS

W. Ruppitsch | A. Stöger | M. Keck

9. NEAR INFRARED SPECTROSCOPY (NIRS): PERSPECTIVE OF FIRE BLIGHT DETECTION IN ASYMPTOMATIC PLANT MATERIAL

F. Spinelli | M. Noferini | G. Costa

10. MONITORING ERWINIA AMYLOVORA IN POME FRUIT ORCHARDS USING REVERSE-DOT-BLOT HYBRIDIZATION

P.L. Sholberg | D. O´Gorman | K.E. Bedford

11. EUROPEAN PROTOCOL FOR DIAGNOSIS OF ERWINIA AMYLOVORA

12. LONG-TERM STARVATION-SURVIVAL OF ERWINIA AMYLOVORA IN STERILE IRRIGATION WATER

E.G. Biosca | E. Marco-Noales | M. Ordax | M.M. Lopez

13. INFLUENCE OF RAIN ON THE TRANSIENT POPULATIONS OF ERWINIA AMYLOVORA ON LEAF SURFACES

S.C. Ockey | S.V. Thomson

14. RELATION OF POMACEOUS FLOWER AGE TO INFECTION BY ERWINIA AMYLOVORA

P.L. Pusey | T.J. Smith

15. SURVIVAL AND GROWTH OF ERWINIA AMYLOVORA ON APPLE LEAVES

J.L. Norelli | M.T. Brandl

16. NECTARY STRUCTURE OF PEAR CULTIVARS AND ITS RELATION TO FIRE BLIGHT SUSCEPTIBILITY

A. Farkas | ZS. Orosz-Kovács | T. Bubán

17. INFLUENCE OF BIOREGULATORS ON APPLE NECTAR CHARACTERISTICS, IN RELATION TO FIREBLIGHT INFECTION

18. ENDOPHYTIC SURVIVAL OF ERWINIA AMYLOVORA IN SYMPTOMLESS PEAR SCIONS

U. Mazzucchi | S. Mucini | F. Traversa | P. Minardi

19. RELATIONSHIPS BETWEEN APIS MELLIFERA AND ERWINIA AMYLOVORA: BIOINDICATION, BACTERIUM DISPERSAL AND QUARANTINE PROCEDURES

20. WEATHER AFFECTING THE INCIDENCE AND SEVERITY OF FIRE BLIGHT IN ENGLAND

E. Billing

21. A STATISTICAL COMPARISON OF MARYBLYT™ AND COUGARBLIGHT WITH SENSITIVITY ANALYSIS

M.M. Dewdney | A.R. Biggs | W.W. Turechek

22. TEN YEAR SUMMARY OF FIRE BLIGHT PREDICTION IN SOUTH-WEST GERMANY (1994-2003)

E. Moltmann

23. COMPARISON OF THE ´COUGARBLIGHT´ AND ´MARYBLYT´ FORECASTING SYSTEMS IN THE PROVINCE OF REGGIO EMILIA (ITALY)

L. Casoli | A. Fontani | A. Montermini | A. Calzolari

24. PREDICTING THE OCCURRENCE OF FIRE BLIGHT USING LEAF WETNESS IN THE SAN JOAQUIN VALLEY OF CALIFORNIA

B.A. Holtz | E.W. Hoffman

25. INTEGRATED FORECASTING SYSTEMS FOR MONITORING FIRE BLIGHT IN TRENTINO

R. Zasso | R. Moskovitch | I. Pertot | C. Gessler

26. MONITORING FIRE BLIGHT ON WILD HOSTS AND ORNAMENTALS

27. COPPER INDUCES A VIABLE BUT NONCULTURABLE (VBNC) STATE IN ERWINIA AMYLOVORA

M. Ordax | E. Marco-Noales | M.M. Lopez | E.G. Biosca

28. PREVENTING SHOOT BLIGHT OF APPLE WITH STREPTOMYCIN APPLICATIONS FOLLOWING SIMULATED HAIL INJURY

S.C. Ockey | S.V. Thomson

29. USING PROHEXADIONE-CALCIUM TO CONTROL FIRE BLIGHT IN YOUNG APPLE TREES

J.R. Norelli | S.S. Miller

30. HOST SUSCEPTIBILITY AND FIRE BLIGHT CONTROL ON APPLE AND PEAR

H. Schoofs | T. Deckers

31. CHEMICAL CONTROL OF FIRE BLIGHT IN PEAR: APPLICATION OF PROHEXADIONE-CALCIUM, ACIBENZOLAR-S-METHYL, AND COPPER PREPARATIONS IN VITRO AND UNDER FIELD CONDITIONS

32. PROHEXADIONE-CALCIUM INDUCES IN APPLE THE BIOSYNTHESIS OF LUTEOFOROL, A NOVEL FLAVAN 4-OL, WHICH IS ACTIVE AGAINST ERWINIA AMYLOVORA

33. GROWTH-REGULATING ACYLCYCLOHEXANEDIONES, TRINEXAPAC-ETHYL AND PROHEXADIONE-CALCIUM DECREASE BLOSSOM BLIGHT INCIDENCE IN POME FRUITS

F. Spinelli | G. Costa | J.L. Vanneste | D.A. Cornish | J. Yu

34. POTATO LEAFHOPPERS, FIRE BLIGHT AND PROHEXADIONE-CA: A LOOK AT THEIR INTERACTION UNDER FIELD CONDITIONS

K. Leahy | D.W. Greene | W.R. Autio | J.L. Norelli

35. MORE RESULTS OF TESTING CONTROL AGENTS FOR FIRE BLIGHT IN THE FIELD WITH A NEW EXPERIMENTAL DESIGN

E. Moltmann | A. Fried | A. Seibold | E. Lange

36. EFFICACIES OF PLANT ACTIVATORS IN CONTROL OF FIRE BLIGHT OF PEAR IN TURKEY

37. AN ATTEMPT TO INCREASE THE EFFICACY OF COPPER COMPOUNDS EMENDED WITH ESSENTIAL OILS AGAINST FIRE BLIGHT

N. Hassanzadeh

38. ERWINIA AMYLOVORA INFECTION OF FLOWERS AND SHOOTS IN APPLE TREES TREATED WITH PROHEXADIONE-CA

39. EVALUATION OF BACTERICIDES FOR CONTROL OF FIRE BLIGHT OF PEARS AND APPLES CAUSED BY ERWINIA AMYLOVORA

40. EFFICACY OF BIOAGENTS AND CHEMICALS AGAINST PEAR SHOOT BLIGHT

C. Bazzi | E. Biondi | R. Berardi | A. Brunelli

41. EPIPHYTIC ERWINIAS TO CONTROL FIRE BLIGHT

V. Jakovljevic | Z. Du | S. Jock | K. Geider

42. COLONIZATION OF FLOWERS BY PSEUDOMONAS FLUORESCENS A506 FORMULATED IN A BIOPOLYMER GEL

V.O. Stockwell | K.B. Johnson | V.W. Johnson

43. BIOAVAILABILITY OF IRON TO PSEUDOMONAS FLUORESCENS STRAIN A506 ON FLOWERS OF PEAR

T.N. Temple | V.O. Stockwell | K.B. Johnson | J.E. Loper

44. ESTABLISHMENT AND SURVIVAL ON APPLE AND PEAR LEAVES OF FOUR BIOLOGICAL CONTROL AGENTS INCLUDING PANTOEA AGGLOMERANS P10C AND PSEUDOMONAS FLUORESCENS A506

J.L. Vanneste | D.A. Cornish | J. Yu | F. Spinelli

45. THE BIOSYNTHETIC GENES OF PANTOCIN A AND PANTOCIN B OF PANTOEA AGGLOMERANS EH318

S.A.I. Wright | M. Jin | J. Clardy | S.V. Beer

46. CLONING FROM PANTOEA AGGLOMERANS PA21889 OF THE GENES INVOLVED IN THE PRODUCTION OF A COMPOUND INHIBITORY TO ERWINIA AMYLOVORA

J.L. Vanneste | D.A. Cornish | M.D. Voyle | D. Koerber | P. Laux

47. REDUCTION OF FIRE BLIGHT INCIDENCE ON APPLE FLOWERS AND COLONISATION OF PEAR SHOOTS IN EXPERIMENTAL ORCHARDS USING PSEUDOMONAS SPP. IPV-BO G19 AND IPV-BO 3371

E. Biondi | C. Bazzi | J.L. Vanneste

48. POTENTIAL AS BIOLOGICAL CONTROL AGENTS OF SOME NOVEL EPIPHYTIC BACTERIAL STRAINS ISOLATED FROM ITALY

C. Bazzi | E. Biondi | J.L. Vanneste

49. STUDIES ON BIOLOGICAL CONTROL OF FIRE BLIGHT WITH SOME ANTAGONISTIC BACTERIA

H. Özaktan | T. Bora

50. BIOCONTROL OF FIRE BLIGHT WITH THE BACTERIAL ANTAGONIST RHANELLA AQUATILIS RA39 IN COMBINATION WITH AROMATIC COMPOUNDS

W. Zeller | P. Laux

51. DEVELOPMENT OF ECO-FRIENDLY STRATEGIES FOR THE CONTROL OF FIRE BLIGHT IN HUNGARY

52. LACK OF REDUCTION OF FIRE BLIGHT INCIDENCE USING NATURAL PRODUCTS WHICH INHIBIT ERWINIA AMYLOVORA IN VITRO

J.L. Vanneste | G. Fischer | A. Spornberger | H.C. Pechhacker | F. Berger

53. BIOCONTROL OF FIRE BLIGHT WITH THE ETHERIC OIL OF THYMBRA SPICATA-BIO ZELL 2000B/AKSEBIO 2

K. Abo-Elyousr | K. Zeller | O. Yegen

54. INVESTIGATIONS ON INDUCED RESISTANCE AGAINST FIRE BLIGHT (ERWINIA AMYLOVORA) WITH DIFFERENT BIOAGENTS

K. Abo-Elyousr | O. Yegen | W. Zeller

55. BIOCONTROL OF ERWINIA AMYLOVORA USING A COMMERCIAL YEAST STRAIN MIXTURE

B. Duffy | J. Vogelsanger | B. Schoch | E. Holliger

56. YEASTS AS ANTAGONISTS AGAINST ERWINIA AMYLOVORA

A. Seibold | M. Viehrig | W. Jelkmann

57. CONTROL OF FIRE BLIGHT WITH A LYSOZYME FROM THE ERWINIA AMYLOVORA PHAGE

EA1HCONTROL OF FIRE BLIGHT WITH A LYSOZYME FROM THE ERWINIA AMYLOVORA PHAGE EA1H

H. Salm | W.S. Kim | K. Geider | B. Schneider

58. BIOCHEMICAL ANALYSES OF POMACEOUS STIGMA EXUDATES AND RELEVANCE TO BIOLOGICAL CONTROL OF FIRE BLIGHT

P.L. Pusey

59. POTENTIAL OF OSMIA CORNUTA AS A CARRIER OF ANTAGONIST BACTERIA IN BIOLOGICAL CONTROL OF FIRE BLIGHT: A COMPARISON WITH APIS MELLIFERA

B. Maccagnani | C. Bazzi | E. Biondi | D. Tesoriero | S. Maini

60. THE STATUS OF FIRE BLIGHT ON PEAR IN EGYPT

A.E. Tawfik | A.F. Abd El-Rahman | E.M. Hussein | N.Y. Abd El-Ghafar

61. SEQUENCING THE ENTIRE GENOME OF ERWINIA AMYLOVORA

S.V. Beer | A.B. Bocsanczy | N.T. Perna | J. Parkhill

62. TWENTY YEARS OF MOLECULAR GENETICS WITH ERWINIA AMYLOVORA: ANSWERS AND NEW QUESTIONS ABOUT EPS-SYNTHESIS AND OTHER VIRULENCE FACTORS

K. Geider

63. EXOPOLYSACCHARIDES OF ERWINIA AMYLOVORA AND RELATED PATHOGENS

64. HRPL NEGATIVELY REGULATES FLAGELLAR SYSTEM IN ERWINIA AMYLOVORA

S. Cesbron | M.N. Brisset | M. Tharaud | J.P. Paulin

65. THE SECRETION OF EOPB FROM ERWINIA AMYLOVORA

J.E. Asselin | C.S. Oh | R.M. Nissinen | S.V. Beer

66. AUTOINDUCTION IN ERWINIA AMYLOVORA

L. Molina | G. Defago | B. Duffy

67. GENETIC ANALYSIS OF THE UBIQUITOUS PLASMID PEA29 AND TWO NEW ERWINIA AMYLOVORA PLASMIDS

G.W. Sundin | G.C. McGhee | G.C. Foster | A.L. Jones

68. AN ERWINIA AMYLOVORA STRAIN WITHOUT PLASMID PEA29 ISOLATED IN A NURSERY FROM HAWTHORN

69. PHENOTYPIC AND GENETIC DIVERSITY OF SELECTED ERWINIA AMYLOVORA STRAINS FROM POLAND

J. Pulawska | K. Kielak | P. Sobiczewski

70. VIRULENCE AND GENOMIC VARIABILITY OF ERWINIA AMYLOVORA STRAINS IN NORTHEASTERN ITALY

P. Minardi | M. Morbio | F. Traversa | U. Mazzucchi

71. THE ANTIBIOTIC SENSITIVITY OF CROATIAN ISOLATES OF ERWINIA AMYLOVORA

E. Dermic | B. Cvjetkovic | D. Dermic

72. PHENOTYPIC CHARACTERIZATION OF ERWINIA AMYLOVORA FROM POME FRUITS IN TURKEY

Y. Aysan | M. Mirik | F. Sahin | R. Kotan | H. Saygili

73. DSPE, AN EFFECTOR OF ERWINIA AMYLOVORA IS TRANSLOCATED INTO PLANT CELLS

A.M. Bocsanczy | R.M. Nissinen | C.S. Oh | S.V. Beer

74. INTERACTION OF DSPE/A, A PATHOGENICITY/AVIRULENCE PROTEIN OF ERWINIA AMYLOVORA, WITH PRE-FERREDOXIN FROM APPLE AND ITS RELATIONSHIP TO PHOTOSYNTHETIC EFFICIENCY

J.M Bonasera | X. Meng | S.V. Beer | T. Owens | W.S. Kim

75. INVOLVEMENT OF THE PHENYLPROPANOID PATHWAY IN THE RESISTANCE OF APPLE TO ERWINIA AMYLOVORA

I. Pontais | J.P. Paulin | M.N. Brisset

76. MECHANISMS INVOLVED IN FIRE BLIGHT PROTECTION OF APPLE WITH AN HRPL MUTANT OF ERWINIA AMYLOVORA

77. PLANT MECHANISMS INTERFERING WITH FIRE BLIGHT INFECTION

M.N. Brisset | J.P. Paulin

78. DIFFERENTIAL EXPRESSION OF PROTEINS IN APPLE FOLLOWING INOCULATION WITH ERWINIA AMYLOVORA

K. Heyens | R. Valcke | D. Dumont | J. Robben | J.P. Noben

79. APPROACH TO THE STUDY OF INDUCTION OF RESISTANCE IN PYRUS COMMUNIS TO E. AMYLOVORA: DEVELOPMENT OF BIOASSAYS AND CLONING OF FRAGMENTS OF NPR1-LIKE GENES

S. Loreti | A. Bosco | A. Gallelli | M. Pilotti | E. Caboni

80. THE FIRE BLIGHT RESISTANCE OF APPLE CLONES IN WHICH DSPE-INTERACTING PROTEINS ARE SILENCED

81. THE NATURE OF RESISTANCE OF THE ´B.9´ APPLE ROOTSTOCK TO FIRE BLIGHT

N. LoGiudice | H.S. Aldwinckle | T.L. Robinson | G. Fazio

82. TRANSGENIC APPLE LINES OVER-EXPRESSING THE APPLE GENE MPNPR1 HAVE INCREASED RESISTANCE TO FIRE BLIGHT

M. Malnoy | M.M. Borejsza-Wysocka | H.S. Aldwinckle | Q.L. Jin | S.Y. HE

83. DIFFERENTIAL SUSCEPTIBILITY TO FIRE BLIGHT IN COMMERCIAL AND EXPERIMENTAL APPLE ROOTSTOCK CULTIVARS

G. Fazio | H.S Aldwinckle | R.P. McQuinn | T.L. Robinson

84. FIRE BLIGHT RESISTANCE IN EXTENSIVE POME FRUIT PRODUCTION IN GERMANY

P. Laux | W. Zeller

85. CONSTITUTIVE EXPRESSION OF ERWINIA AMYLOVORA HRPN IN TRANSGENIC PEARS REDUCES FIRE BLIGHT SUSCEPTIBILITY

E. Chevreau | M. Malnoy | M. Boccara

86. ENGINEERING IMPROVED RESISTANCE AGAINST THE FIRE BLIGHT PATHOGEN IN APPLE CULTIVARS ´ELSTAR´ AND ´ROYAL GALA´ BY EXPRESSION OF HUMAN LACTOFERRIN

B. Schneider | C. Berwarth | W. Jelkmann

87. FIRST RESULTS OF FIRE BLIGHT RESISTANCE SCREENING IN A HUNGARIAN APPLE BREEDING PROGRAMME

M. Toth | M. Kasa | M. Göndör | K Honty | M. Hevesi

88. FIRE BLIGHT SUSCEPTIBILITY OF NEW APPLE CULTIVARS AND CLONES FROM POLAND

P. Sobiczewski | E. Żurawicz | S. Berczyński | M. Lewandowski

89. SEARCH FOR MOLECULAR MARKERS LINKED TO FIRE BLIGHT RESISTANCE IN PEAR (PYRUS COMMUNIS)

M.R. Afunian | P.H. Goodwin | D.M. Hunter

91. REACTIVITY TO FIRE BLIGHT OF NEW PROMISING PEAR SELECTIONS

92. EVALUATION OF RESISTANCE TO FIRE BLIGHT IN CZECH PEAR CULTIVARS

F. Paprstein | J. Kosina | J. Korba | J. Sillerova

93. SUSCEPTIBILITY OF PEAR CULTIVARS TO FIRE BLIGHT IN HUNGARY

K. Honty | M. Göndör | M. Toth | K Kasa | M. Hevesi