Articles
EFFECT OF DONOR PLANT PHYSIOLOGICAL CONDITION ON IN VITRO ESTABLISHMENT OF VACCINIUM ANGUSTIFOLIUM SHOOT EXPLANTS
Article number
715_65
Pages
433 – 438
Language
English
Abstract
Culture establishment for in vitro propagation is strongly influenced by the physiological condition of the donor plant serving as the explant source.
The aim of these experiments was to identify suitable periods and developmental stages of donor plant for Vaccinium angustifolium explant establishment. In order to develop a well functioning protocol for culture initiation, the explants were harvested at three different dates: in February when plants in the field are dormant, in May when vegetation period is about to start and in August after the crop has been harvested.
The influence of pretreatment (forcing indoors for 7-14 days) on dormant plants before explant collection was investigated.
Single node explants from field grown lowbush blueberry plants were used for culture establishment on modified WPM medium supplemented with zeatin at 4.56 μM. The rate of contamination, viability of explants and number of shoots per explant were estimated after 2 and 6 weeks in culture.
The best results were obtained with explants collected from pre-treated plants in May.
The contamination rate was low and after 6 weeks in culture, 98% of the explants survived and 94% had developed shoots >10mm.
The explants collected in February from both dormant and pre-treated plants had high contamination rates and more died during first weeks in culture.
The effect of cytokinin 1-phenyl-3-(1,2,3-thidiazol-5-yl) urea (thidiazuron, TDZ) at concentrations 0.045, 0.91, 2.72 and 4.54 μM on explant development and shoot proliferation was investigated.
At all TDZ concentrations tested the media promoted callus development and shoots developed remained significantly shorter than on the control media supplemented with zeatin at 4,56 μM. Increase in fresh weight was the highest at TDZ concentrations 0.91, 2.72 and 4.54 μM but shoot proliferation rate on all TDZ supplemented media remained significantly lower than that of the control.
The aim of these experiments was to identify suitable periods and developmental stages of donor plant for Vaccinium angustifolium explant establishment. In order to develop a well functioning protocol for culture initiation, the explants were harvested at three different dates: in February when plants in the field are dormant, in May when vegetation period is about to start and in August after the crop has been harvested.
The influence of pretreatment (forcing indoors for 7-14 days) on dormant plants before explant collection was investigated.
Single node explants from field grown lowbush blueberry plants were used for culture establishment on modified WPM medium supplemented with zeatin at 4.56 μM. The rate of contamination, viability of explants and number of shoots per explant were estimated after 2 and 6 weeks in culture.
The best results were obtained with explants collected from pre-treated plants in May.
The contamination rate was low and after 6 weeks in culture, 98% of the explants survived and 94% had developed shoots >10mm.
The explants collected in February from both dormant and pre-treated plants had high contamination rates and more died during first weeks in culture.
The effect of cytokinin 1-phenyl-3-(1,2,3-thidiazol-5-yl) urea (thidiazuron, TDZ) at concentrations 0.045, 0.91, 2.72 and 4.54 μM on explant development and shoot proliferation was investigated.
At all TDZ concentrations tested the media promoted callus development and shoots developed remained significantly shorter than on the control media supplemented with zeatin at 4,56 μM. Increase in fresh weight was the highest at TDZ concentrations 0.91, 2.72 and 4.54 μM but shoot proliferation rate on all TDZ supplemented media remained significantly lower than that of the control.
Authors
H. Kaldmäe, M. Starast, K. Karp, T. Paal
Keywords
Vaccinium angustifolium, in vitro, propagation, thidiazuron
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