Articles
CLONING AND MAPPING OF RESISTANCE GENE HOMOLOGUES IN APRICOT (PRUNUS ARMENIACA L.)
Article number
717_22
Pages
115 – 118
Language
English
Abstract
Genomic DNA sequences, sharing homology with the NBS-LRR (Nucleotide Binding Site – Leucine Rich Repeat) resistance genes, were isolated and cloned from apricot using a PCR approach with degenerate primers designed from conserved regions of the NBS domain.
RGA-specific primers were developed from the consensus sequences of each apricot RGA family and were used to develop AFLP-RGA markers by means of an AFLP-modified procedure, where one standard primer is substituted by an RGA-specific primer.
Using this method, 27 polymorphic markers, six of which shared homology with the TIR class of the NBS-LRR R-genes, were obtained from 17 different primer combinations.
Sixteen of the 27 mapped in an apricot genetic map previously constructed from the self-pollination of the cultivar Lito.
RGA-specific primers were developed from the consensus sequences of each apricot RGA family and were used to develop AFLP-RGA markers by means of an AFLP-modified procedure, where one standard primer is substituted by an RGA-specific primer.
Using this method, 27 polymorphic markers, six of which shared homology with the TIR class of the NBS-LRR R-genes, were obtained from 17 different primer combinations.
Sixteen of the 27 mapped in an apricot genetic map previously constructed from the self-pollination of the cultivar Lito.
Authors
J.M. Soriano, C. Romero, S. Vilanova, G. LJacer, M.L. Badenes
Keywords
NBS-LRR sequences, RGAs, apricot
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