EFFECTS OF SILVER NITRATE, ACTIVATED CHARCOAL AND COLD TREATMENT ON THE IN VITRO ANDROGENESIS OF PEPPER (CAPSICUM ANNUUM L.)

The influence of cold-shock treatment of Capsicum annuum L. buds at 4°C for 48 h or 96 h, the additions of different concentrations of silver nitrate (AgNO₃) (0, 1, 5 and 10 mg L^-1) and 0.25% activated charcoal to the culture medium on pepper androgenesis were evaluated.
Floral buds were collected whose anthers contained uninucleate microspores.
Murashige and Skoog (MS) medium supplemented with combinations of 4 mg L^-1 naphthalene acetic acid (NAA) + 1 mg L^-1 benzyladenine (BA) or 1 mg L^-1 NAA + 4 mg L^-1 BA 0.8% (w/v) agar and 3% (w/v) sucrose.
The highest numbers of embryos (12.5 embryos/100 anthers) were obtained from the control anthers which were not subjected to cold treatments and cultured on MS medium containing 4 mg L^-1 NAA and 1 mg L^-1 BA and activated charcoal without AgNO₃.

III Balkan Symposium on Vegetables and Potatoes

D. Özkum, R. Tıpırdamaz

anther culture, Solanaceae, AgNO₃, cold pretreatment, embryo formation, haploid plants, culture media

https://doi.org/10.17660/ActaHortic.2007.729.20