Articles
PRODUCTION OF TRANSGENIC PHALAENOPSIS PLANTS BY INTRODUCING GLUTATHIONE S-TRANSFERASE GENE INTO PROTOCORMS AT AN EARLY STAGE AFTER GERMINATION
Article number
743_13
Pages
101 – 105
Language
English
Abstract
Transgenic Phalaenopsis plants over-expressing glutathione S-transferase (GST) gene were produced by Agrobacterium-mediated transformation method.
Protocorms at an early stage after germination were inoculated with A. tumefaciens strain EHA101 (pEKH35S163P) that harbors GST, hygromycin phosphotransferase and neomycin phosphotransferase II genes.
A total of 68 independent transgenic plants derived from 6325 mature seeds were obtained 6 months after infection with Agrobacterium. Regenerated plants were grown in greenhouse and all plants bloomed within 2 years.
Five months after pollination, 13 seed pods were obtained from 6 plants showing suitable commercial characters.
Germinated Phalaenopsis T1 seedlings with GST gene were confirmed by PCR analysis.
Northern blot analysis confirmed the expression of GST in hygromycin-resistant plantlets.
Protocorms at an early stage after germination were inoculated with A. tumefaciens strain EHA101 (pEKH35S163P) that harbors GST, hygromycin phosphotransferase and neomycin phosphotransferase II genes.
A total of 68 independent transgenic plants derived from 6325 mature seeds were obtained 6 months after infection with Agrobacterium. Regenerated plants were grown in greenhouse and all plants bloomed within 2 years.
Five months after pollination, 13 seed pods were obtained from 6 plants showing suitable commercial characters.
Germinated Phalaenopsis T1 seedlings with GST gene were confirmed by PCR analysis.
Northern blot analysis confirmed the expression of GST in hygromycin-resistant plantlets.
Publication
Authors
D.P. Chin, M. Mii, K.I. Mishiba
Keywords
Agrobacterium tumefaciens, genetic transformation, orchid, over-expression, stress
Online Articles (19)