Articles
INTERSPECIFIC HYBRIDIZATION BETWEEN KALANCHOE BLOSSFELDIANA AND SEVERAL WILD KALANCHOE SPECIES WITH ORNAMENTAL VALUE
Article number
743_7
Pages
59 – 65
Language
English
Abstract
Kalanchoe blossfeldiana (sect.
Kalanchoe) is one of the most important ornamental indoor plants during the winter season.
Breeding of K. blossfeldiana has been restricted to intra and interspecific hybridization with allied species.
To enlarge the genetic variability, reciprocal crosses were carried out between K. blossfeldiana and several wild Kalanchoe species which belong to sect.
Kalanchoe and sect.
Bryophyllum, and ovule culture technique was applied to rescue the hybrid embryos.
Hybridity of the F1 plants thus obtained was confirmed by flow cytometric and RAPD analyses.
Consequently, 6 intra-section and 3 inter-section cross combinations were obtained in the present study.
Reciprocal compatibility was observed in the crosses with 2 wild species.
All the other hybrids could be obtained when K. blossfeldiana was used as maternal donor.
Flower morphology and color of these hybrids were almost intermediate between both parental species.
Since these F1 hybrids were sterile, amphidiploids of these hybrids were produced to restore the fertility by utilizing spontaneous chromosome doubling in tissue culture.
Kalanchoe) is one of the most important ornamental indoor plants during the winter season.
Breeding of K. blossfeldiana has been restricted to intra and interspecific hybridization with allied species.
To enlarge the genetic variability, reciprocal crosses were carried out between K. blossfeldiana and several wild Kalanchoe species which belong to sect.
Kalanchoe and sect.
Bryophyllum, and ovule culture technique was applied to rescue the hybrid embryos.
Hybridity of the F1 plants thus obtained was confirmed by flow cytometric and RAPD analyses.
Consequently, 6 intra-section and 3 inter-section cross combinations were obtained in the present study.
Reciprocal compatibility was observed in the crosses with 2 wild species.
All the other hybrids could be obtained when K. blossfeldiana was used as maternal donor.
Flower morphology and color of these hybrids were almost intermediate between both parental species.
Since these F1 hybrids were sterile, amphidiploids of these hybrids were produced to restore the fertility by utilizing spontaneous chromosome doubling in tissue culture.
Publication
Authors
Y. Izumikawa, I. Nakamura, M. Mii
Keywords
pollination, ovule culture, flow cytometry, RAPD analysis, polyploidization, amphidiploid
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