Articles
GISH ANALYSIS OF HYBRIDS PRODUCED BY INTERSPECIFIC HYBRIDIZATION BETWEEN TULIPA GESNERIANA AND T. FOSTERIANA
Article number
743_18
Pages
133 – 137
Language
English
Abstract
Genomic in situ hybridization (GISH) was used to understand the process of interspecific tulip hybrid diploid (2n = 2x = 24), triploid (2n = 3x = 36) and tetraploid (2n = 4x = 48) cultivar formation.
Simultaneous hybridization of differentially labelled genomic probes enabled chromosomes derived from T. gesneriana and T. fosteriana to be distinguished in all cultivars tested.
Diploid cultivar Shirayukihime contained one set of chromosomes from T. gesneriana and one set from T. fosteriana. In triploid cultivars Come Back, Diplomate, Pink Impression and Oxford, 24 chromosomes were derived from T. gesneriana and 12 chromosomes originated from T. fosteriana. The genome of tetraploid cultivar Ollioules comprised 36 chromosomes from T. gesneriana and 12 chromosomes from T. fosteriana. In all tulip hybrids, GISH signals were localized predominately in telomeric/subtelomeric and intercalary regions of T. gesneriana chromosomes, whereas T. fosteriana chromosomes were painted more evenly along the chromosome length.
In triploid and tetraploid cultivars, cross-hybridization to putative rRNA gene sites was observed at telomeric and intercalary locations of some chromosomes, although additional experiments are needed to identify what kind of repetitive DNA families are arranged as nested elements interspersed in the tulip genome.
Simultaneous hybridization of differentially labelled genomic probes enabled chromosomes derived from T. gesneriana and T. fosteriana to be distinguished in all cultivars tested.
Diploid cultivar Shirayukihime contained one set of chromosomes from T. gesneriana and one set from T. fosteriana. In triploid cultivars Come Back, Diplomate, Pink Impression and Oxford, 24 chromosomes were derived from T. gesneriana and 12 chromosomes originated from T. fosteriana. The genome of tetraploid cultivar Ollioules comprised 36 chromosomes from T. gesneriana and 12 chromosomes from T. fosteriana. In all tulip hybrids, GISH signals were localized predominately in telomeric/subtelomeric and intercalary regions of T. gesneriana chromosomes, whereas T. fosteriana chromosomes were painted more evenly along the chromosome length.
In triploid and tetraploid cultivars, cross-hybridization to putative rRNA gene sites was observed at telomeric and intercalary locations of some chromosomes, although additional experiments are needed to identify what kind of repetitive DNA families are arranged as nested elements interspersed in the tulip genome.
Publication
Authors
A. Marasek, K. Okazaki
Keywords
genomic in situ hybridization, cultivar origin, tulip
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