Articles
GENETIC RELATIONSHIPS AMONG NATIVE AND INTRODUCED RUBUS SPECIES IN JAPAN BASED ON RBCL SEQUENCE
Article number
769_26
Pages
195 – 199
Language
English
Abstract
Many Rubus species are native to Japan, but no cultivars have been bred from them.
To determine genetic relationships among wild Rubus species native to Japan and cultivars which were introduced from foreign countries, chloroplast DNA sequences were investigated and the phylogeny determined.
Ten native species and fifteen cultivars were investigated.
DNA sequences of the chloroplast gene rbcL were examined to construct the phylogeny of these species. Malus × domestica was used as an out-group.
Aligned sequences of rbcL from the 39 plants resulted in 1296 characters.
According to the phylogram, five clusters were classified.
The clusters were separated into three groups: mixed native Japanese species and introduced cultivars, only cultivars, and only native species.
Clusters I and III consisted of native species and cultivars.
Cluster I comprised of Rubus pectinellus, a native species, cultivars of R. occidentalis, which were considered to be a black raspberry, and Brandywine a Rubus hybrid, which was considered to be a purple raspberry.
In cluster III, two native species, R. vernus and R. ikenoensis belonged to the same group as all the cultivars of R. idaeus used in this study, which were considered to be a red raspberry.
Cluster II was composed of six cultivars, Trailblazer (R. strigosus), which was considered to be a red raspberry, Burbank Thornless (R. ursinus) and four cultivars of R. hybrid, which were considered to be a blackberry.
Four of the native species, R. palmatus, R. crataegifolius, R. trifidus and R. hirsutus belonged to cluster IV. Cluster V, which was distantly related to the other clusters, consisted of three native species, R. parvifolius, R. phoenicolasius and R. mesogaeus. These results provide basic information on phylogeny, taxonomy and breeding of Rubus species.
To determine genetic relationships among wild Rubus species native to Japan and cultivars which were introduced from foreign countries, chloroplast DNA sequences were investigated and the phylogeny determined.
Ten native species and fifteen cultivars were investigated.
DNA sequences of the chloroplast gene rbcL were examined to construct the phylogeny of these species. Malus × domestica was used as an out-group.
Aligned sequences of rbcL from the 39 plants resulted in 1296 characters.
According to the phylogram, five clusters were classified.
The clusters were separated into three groups: mixed native Japanese species and introduced cultivars, only cultivars, and only native species.
Clusters I and III consisted of native species and cultivars.
Cluster I comprised of Rubus pectinellus, a native species, cultivars of R. occidentalis, which were considered to be a black raspberry, and Brandywine a Rubus hybrid, which was considered to be a purple raspberry.
In cluster III, two native species, R. vernus and R. ikenoensis belonged to the same group as all the cultivars of R. idaeus used in this study, which were considered to be a red raspberry.
Cluster II was composed of six cultivars, Trailblazer (R. strigosus), which was considered to be a red raspberry, Burbank Thornless (R. ursinus) and four cultivars of R. hybrid, which were considered to be a blackberry.
Four of the native species, R. palmatus, R. crataegifolius, R. trifidus and R. hirsutus belonged to cluster IV. Cluster V, which was distantly related to the other clusters, consisted of three native species, R. parvifolius, R. phoenicolasius and R. mesogaeus. These results provide basic information on phylogeny, taxonomy and breeding of Rubus species.
Authors
H. Imanishi, K. Nakahara, H. Tsuyuzaki
Keywords
cultivar, chloroplast, DNA, phylogeny, raspberry, blackberry
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