Articles
IDENTIFICATION AND CHARACTERIZATION OF GIBBERELLIN-RELATED DWARF MUTANTS IN MALUS HUPEHENSIS
Article number
774_4
Pages
41 – 48
Language
English
Abstract
Five dwarf mutants, A1, A1D, B1, C2, and C3 selected from 
-rays radiated ‘Pingyitiancha’ Malus hupehensis seeds were treated with exogenous gibberellic acid (GA3), auxin (NAA) and brassinolide (BL). Endogenous GA3 and GA4 content in both the wild type and the mutants were measured in the rapid shoot growing period with an enzymelinked immunosorbent assay (ELISA). The results showed that the mutants B1, A1, A1D, C2, and C3 exhibited reduced responses to exogenous GA3 compared to that of wild type.
Content of GA3 was 2 to 6-fold more and GA4 was 6 to 60-fold more in the leaves of the mutants than in leaves of the wild type ‘Pingyitiancha’. This indicated that they were GA-response mutants.
-rays radiated ‘Pingyitiancha’ Malus hupehensis seeds were treated with exogenous gibberellic acid (GA3), auxin (NAA) and brassinolide (BL). Endogenous GA3 and GA4 content in both the wild type and the mutants were measured in the rapid shoot growing period with an enzymelinked immunosorbent assay (ELISA). The results showed that the mutants B1, A1, A1D, C2, and C3 exhibited reduced responses to exogenous GA3 compared to that of wild type.Content of GA3 was 2 to 6-fold more and GA4 was 6 to 60-fold more in the leaves of the mutants than in leaves of the wild type ‘Pingyitiancha’. This indicated that they were GA-response mutants.
Authors
Guangli Sha, Xianghui Gong, Yanjing Shi, Yongchun Shao, Tao Yin, Yujin Hao, Huairui Shu
Keywords
gibberellin, GA-response mutant, Malus hupehensis, ELISA
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