Articles
EFFECTS OF ETHEPHON TREATMENT ON THE SOLUBLE PROTEIN, ISOENZYMES, AND THEIR ACTIVITIES IN MUSKMELON (CUCUMIS MELO L. VAR. RETICULATES NAUD.)
Article number
774_45
Pages
329 – 338
Language
English
Abstract
Muskmelons (Cucumis melo L. var. reticulatus Naud.) set fruits on primary branches, but can also be induced to produce fruits on the main vine by ethephon treatment.
In the present paper, the effects of ethephon on the soluble proteins, isozymes, and their activity were studied in muskmelon seedlings of Chinese native muskmelon Xi Yu No.1. Ethephon solution was sprayed on seedlings at a concen¬tration of 150 mg/L while pure water was used as control.
The results indicated that the soluble protein contents increased after ethephon treatment, and there were four new protein bands emerging after 72 h treatment with Rf 0.33, 0.55, 0.57, and 0.60 respectively, which indicated higher soluble protein content and that the specific proteins could enhanced the differentiation of female flowers in muskmelon.
The activity and enzyme of POD increased after ethephone treatment, and three new POD isozyme bands were found between 48 and 72 h of treatment, at Rf 0.55, 0.61 and 0.68. However, one isozyme at Rf 0.68 disappeared between 96 and 120 h after treatment.
Meanwhile, the activities of CAT as well as the isozyme bands of esterase were reduced, whereas the activities of PPO and SOD and their isozymes were not influenced.
From above results, it could be concluded that higher activity and more isozymes of POD showed positive relation with formation of female flowers.
On the contrary, activities of CAT and EST showed negative relationships with female flower formation, while the activities of SOD and PPO had no correlation.
In the present paper, the effects of ethephon on the soluble proteins, isozymes, and their activity were studied in muskmelon seedlings of Chinese native muskmelon Xi Yu No.1. Ethephon solution was sprayed on seedlings at a concen¬tration of 150 mg/L while pure water was used as control.
The results indicated that the soluble protein contents increased after ethephon treatment, and there were four new protein bands emerging after 72 h treatment with Rf 0.33, 0.55, 0.57, and 0.60 respectively, which indicated higher soluble protein content and that the specific proteins could enhanced the differentiation of female flowers in muskmelon.
The activity and enzyme of POD increased after ethephone treatment, and three new POD isozyme bands were found between 48 and 72 h of treatment, at Rf 0.55, 0.61 and 0.68. However, one isozyme at Rf 0.68 disappeared between 96 and 120 h after treatment.
Meanwhile, the activities of CAT as well as the isozyme bands of esterase were reduced, whereas the activities of PPO and SOD and their isozymes were not influenced.
From above results, it could be concluded that higher activity and more isozymes of POD showed positive relation with formation of female flowers.
On the contrary, activities of CAT and EST showed negative relationships with female flower formation, while the activities of SOD and PPO had no correlation.
Authors
Z. Wu, R.M. Song
Keywords
ethephon, muskmelon, protein, isozyme, enzyme activity
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