Articles
SELECTION OF 5-METHYLTRYPTOPHAN-RESISTANT ASPARAGUS WITH ETHYL METHANESULFONATE TREATMENT
Article number
776_43
Pages
333 – 336
Language
English
Abstract
Immediately after pollination female flowers of asparagus plants were treated with ethyl methanesulfonate for selection of mutants resistant to 5-methyltryptophan (5MT), and seeds (M1) were obtained after 3 months.
The M1 seeds were lighter and showed a lower germination rate respect to control seeds.
Various morphological mutations were observed in the M1 seedlings and many of them had longer cladophylls and shorter internodes than control plants.
Meristems originating from the M1 plants were cultured on medium containing 5MT. Selection based on the shoot growth index was carried out after 3 weeks, and a few 5MT-resistant lines were selected among culture lines.
The M1 seeds were lighter and showed a lower germination rate respect to control seeds.
Various morphological mutations were observed in the M1 seedlings and many of them had longer cladophylls and shorter internodes than control plants.
Meristems originating from the M1 plants were cultured on medium containing 5MT. Selection based on the shoot growth index was carried out after 3 weeks, and a few 5MT-resistant lines were selected among culture lines.
Publication
Authors
T. Sonoda, Y. Hayashi, A. Kanno, T. Kameya
Keywords
Asparagus officinalis L., mutant, selection, 5-methyltryptophan-resistant, ethyl methanesulfonate
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