Articles
HIBISCUS ROSA SINENSIS L.: AFLP MARKERS FOR GENETIC IMPROVEMENT
Article number
788_10
Pages
97 – 102
Language
English
Abstract
Commercial varieties of Hibiscus rosa sinensis came from the interspecific crossing work carried out in the past, involving tropical and subtropical species: H. arnottianus, H. kokio, H. denisonii and other unknown plant sources.
This explains the great variability expressed in morphological and agronomical features.
The use of molecular markers in ornamental plant breeding is very considerable for several applications.
Amplified Fragment Length Polymorphisms (AFLP) technique was used with the aim to characterize a collection of commercial varieties of H. rosa sinensis and to determine genetic similarities within them.
Total 64 varieties of H. rosa sinensis and four genotypes of H. syriacus, included to assess the efficiency of AFLP method, were analyzed with eight selected primer combinations.
All cultivars were clearly distinguished by their molecular fingerprints; a total of 213 polymorphic bands were detected and used to construct a similarity matrix by means of Jaccard coefficient.
The Unweighted Pair Group Method with Arithmetic Average (UPGMA) derived tree separated the cultivars into two main branches: the first one included all H. rosa sinensis samples, the second one properly grouped H. syriacus genotypes.
Several sub-clusters were observed.
This confirmed the large genetic variability of our H. rosa sinensis germplasm collection.
The AFLP markers applied in a larger genotype assortment could be useful to perform a more efficient breeding design.
This explains the great variability expressed in morphological and agronomical features.
The use of molecular markers in ornamental plant breeding is very considerable for several applications.
Amplified Fragment Length Polymorphisms (AFLP) technique was used with the aim to characterize a collection of commercial varieties of H. rosa sinensis and to determine genetic similarities within them.
Total 64 varieties of H. rosa sinensis and four genotypes of H. syriacus, included to assess the efficiency of AFLP method, were analyzed with eight selected primer combinations.
All cultivars were clearly distinguished by their molecular fingerprints; a total of 213 polymorphic bands were detected and used to construct a similarity matrix by means of Jaccard coefficient.
The Unweighted Pair Group Method with Arithmetic Average (UPGMA) derived tree separated the cultivars into two main branches: the first one included all H. rosa sinensis samples, the second one properly grouped H. syriacus genotypes.
Several sub-clusters were observed.
This confirmed the large genetic variability of our H. rosa sinensis germplasm collection.
The AFLP markers applied in a larger genotype assortment could be useful to perform a more efficient breeding design.
Publication
Authors
S. Bruna, L. Braglia, V. Casabianca, A. Mercuri, L. de Benedetti, T. Schiva
Keywords
hibiscus cultivars, molecular markers, genetic variability, ornamental breeding
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