Articles
CULTIVAR IDENTIFICATION AND ELUCIDATION OF GENETIC RELATIONSHIPS WITHIN THE SPECIES OLEA EUROPAEA L. USING MICROSATELLITES
Article number
791_9
Pages
79 – 86
Language
English
Abstract
The genetic knowledge about the species Olea europaea L. and the evolutionary relationships among the most extensively employed cultivars is limited.
The purpose of this report is to test the suitability of 15 available microsatellites for identifying 51 olive cultivars and elucidating the phylogenetic relationships between them.
All cultivars except one pair (‘Cornicabra’-‘Morisca’) could be unequivocally identified using only three microsatellites, thanks to the important presence of unique alleles (10 out of 38 in those three microsatellites and 39 out of 122 when 15 markers were considered) and genotypes (43 out of 63 in those three microsatellites and 103 out of 183 for the complete set of primers), but it was necessary to employ an additional one to discriminate between the two mentioned cultivars.
The discrimination power of every microsatellite was calculated in order to evaluate their efficiency in cultivar identification.
Microsatellites with discrimination power value higher than 0.8 resulted especially useful for distinguishing cultivars.
The data coming from genotyping the 51 cultivars with the 15 microsatellites were used for constructing a dendrogram by UPGMA cluster analysis using the Dice similarity coefficient.
Cultivars association according to their geographical origin was observed, showing clearly in the case of the Spanish varieties.
The purpose of this report is to test the suitability of 15 available microsatellites for identifying 51 olive cultivars and elucidating the phylogenetic relationships between them.
All cultivars except one pair (‘Cornicabra’-‘Morisca’) could be unequivocally identified using only three microsatellites, thanks to the important presence of unique alleles (10 out of 38 in those three microsatellites and 39 out of 122 when 15 markers were considered) and genotypes (43 out of 63 in those three microsatellites and 103 out of 183 for the complete set of primers), but it was necessary to employ an additional one to discriminate between the two mentioned cultivars.
The discrimination power of every microsatellite was calculated in order to evaluate their efficiency in cultivar identification.
Microsatellites with discrimination power value higher than 0.8 resulted especially useful for distinguishing cultivars.
The data coming from genotyping the 51 cultivars with the 15 microsatellites were used for constructing a dendrogram by UPGMA cluster analysis using the Dice similarity coefficient.
Cultivars association according to their geographical origin was observed, showing clearly in the case of the Spanish varieties.
Publication
Authors
A. Diaz, A. Martín, R. de la Rosa, P. Rallo
Keywords
microsatellites, cultivar identification, Olea europaea L., phylogeny
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