Articles
EFFECTS OF TEMPERATURE ON POLLEN VIABILITY AND FLOWER OPENING IN WARATAH (TELOPEA SPECIOSISSIMA R.BR.)
Article number
805_12
Pages
99 – 106
Language
English
Abstract
Using blooms on large pot-grown waratahs (Telopea speciosissima R.Br, family Proteaceae), the effects of temperature on pollen viability and flower maturation were examined.
Pollen viability was estimated by germination in 10% sucrose and 100 mg/L boron or staining with fluorescein diacetate (FDA). The rate of flower opening varied with the temperature at which plants or flowers were held; the lower the temperature the slower the rate of flower opening.
Air temperatures equal to or higher than 28°C significantly decreased pollen viability on the inflorescence.
Collected pollen with a moisture content of 17% was stored for 24 hours at 5, -20 and -190°C resulting in respective viability percentages of 65, 81, 72% of pre-storage viability.
Pollen viability was estimated by germination in 10% sucrose and 100 mg/L boron or staining with fluorescein diacetate (FDA). The rate of flower opening varied with the temperature at which plants or flowers were held; the lower the temperature the slower the rate of flower opening.
Air temperatures equal to or higher than 28°C significantly decreased pollen viability on the inflorescence.
Collected pollen with a moisture content of 17% was stored for 24 hours at 5, -20 and -190°C resulting in respective viability percentages of 65, 81, 72% of pre-storage viability.
Publication
Authors
C.A. Offord
Keywords
breeding, Proteaceae, Australia, pollen storage, hybridisation, fluorescein diacetate
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