Articles
RESISTANCE OF IN VITRO GROWN FORAGE CACTUS CLONES TO DACTYLOPIUS OPUNTIAE (HEMIPTERA: DACTYLOPIIDAE)
Article number
811_40
Pages
299 – 302
Language
English
Abstract
The carmine cochineal is an important plague that attacks forage cactus plantations in Brazilian Northeast.
Insect survival and development of carmine cochineal Dactylopius opuntiae (Hemiptera: Dactylopiidae) was studied in different in vitro micropropagated clones of forage cactus.
In this experiment, the development of carmine cochineal colonies, originated from laboratory multiplied nymphs, was statistically evaluated as a randomized block design, with five treatments: gigante, miúda, orelha de elefante mexicana, algerian and F-263 clones, with 12 repetitions.
Each repetition consisted of one plant at six days of age.
For the variance analysis, the data were transformed into square root of %+0.5. The plants, removed from culture medium, were transferred to 200 ml glass containers using filter paper.
These plants were artificially infestated with 10 crawler nymphs which remained confined until their adult phase.
It was observed variation of the resistance among clones when the Tukey test (P<0.05) for comparison of the averages was applied.
No insect development was observed on miúda and orelha de elefante mexicana clones, suggesting a resistance of immunity type or antixenosis.
The results indicate that in vitro micropropagated clones of forage cactus differ among themselves in terms of resistance of carmine cochineal attack.
Insect survival and development of carmine cochineal Dactylopius opuntiae (Hemiptera: Dactylopiidae) was studied in different in vitro micropropagated clones of forage cactus.
In this experiment, the development of carmine cochineal colonies, originated from laboratory multiplied nymphs, was statistically evaluated as a randomized block design, with five treatments: gigante, miúda, orelha de elefante mexicana, algerian and F-263 clones, with 12 repetitions.
Each repetition consisted of one plant at six days of age.
For the variance analysis, the data were transformed into square root of %+0.5. The plants, removed from culture medium, were transferred to 200 ml glass containers using filter paper.
These plants were artificially infestated with 10 crawler nymphs which remained confined until their adult phase.
It was observed variation of the resistance among clones when the Tukey test (P<0.05) for comparison of the averages was applied.
No insect development was observed on miúda and orelha de elefante mexicana clones, suggesting a resistance of immunity type or antixenosis.
The results indicate that in vitro micropropagated clones of forage cactus differ among themselves in terms of resistance of carmine cochineal attack.
Authors
D.M. Passos da Silva, L.M. Houllou-Kido, D. Cordeiro dos Santos, R. Gonçalves Ferreira, V. Felipe dos Santos, W. Melo Ferreira, M. Silva de Lima, H. Marinho Falcão, F. de Sena Tabosa
Keywords
carmine cochineal, tissue culture, Nopalea cochenillifera, plague
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