Articles
EFFECTS OF PRETREATMENT WITH GIBBERELLIC ACID (GA3) AND PROMALIN (GA4 +GA7 +BA) ON GERMINATION OF PROTEA ARISTATA AND P. REPENS
Article number
813_57
Pages
441 – 444
Language
English
Abstract
Dormancy in Protea spp. seeds (botanically achenes) seems to be mainly imposed by a low temperature requirement.
In order to study the effects of gibberellic acid (GA3) and promalin (GA4 +GA7 +BA) on germination of Protea aristata and P. repens, seeds of those species were sown in a mixture of peat moss and lapilli (1:1 in volume). Before sowing, seeds were imbibed for 24h in GA3 (100 ppm), or promalin (100, 200, 400 ppm) or distilled water (control). Germination was recorded weekly, for 15 weeks.
Percentages and rates of germination were calculated.
In P. aristata, pretreatment with 100 ppm of GA3 gave the highest percentage of germination (62.5%) followed by pretreatment with 100 ppm of promalin (47.5%) and pretreatment control (47.5%). There were no significant differences among those pretreatments.
Increasing promalin concentration decreased germination percentages, although not significantly.
As for germination rates, there were no significant differences among pretreatments.
Although a tetrazolium test of P. repens seeds showed that 58% were viable, germination percentages were abnormally low, perhaps due to incubation of the seeds under a no-optimal temperature regime.
In order to study the effects of gibberellic acid (GA3) and promalin (GA4 +GA7 +BA) on germination of Protea aristata and P. repens, seeds of those species were sown in a mixture of peat moss and lapilli (1:1 in volume). Before sowing, seeds were imbibed for 24h in GA3 (100 ppm), or promalin (100, 200, 400 ppm) or distilled water (control). Germination was recorded weekly, for 15 weeks.
Percentages and rates of germination were calculated.
In P. aristata, pretreatment with 100 ppm of GA3 gave the highest percentage of germination (62.5%) followed by pretreatment with 100 ppm of promalin (47.5%) and pretreatment control (47.5%). There were no significant differences among those pretreatments.
Increasing promalin concentration decreased germination percentages, although not significantly.
As for germination rates, there were no significant differences among pretreatments.
Although a tetrazolium test of P. repens seeds showed that 58% were viable, germination percentages were abnormally low, perhaps due to incubation of the seeds under a no-optimal temperature regime.
Authors
J.A. Rodríguez-Pérez, A.M. de León-Hernández, M.C. Vera-Batista, I. Rodríguez-Hernández, P. Alberto-Rodríguez
Keywords
Protea, promalin, gibberellic acid, germination
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