Articles
MICROPROPAGATION OF AN AMAZONIAN TERRESTRIAL ORCHID (BRASSIA BIDDENS) FROM RORAIMA STATE, BRAZIL
Article number
813_60
Pages
459 – 464
Language
English
Abstract
There are about 2,500 of native orchids in Brazilian States, especially in Roraima and Amazonas.
The burning and logging has caused devastation of the Amazon rainforest and loss of species, including orchids.
Tissue culture has proven to be a valuable tool to increase production and preserve orchid species.
Orchids have been multiplied routinely by seeds using a non-symbiotic culture as a propagation method.
The objective of this paper was to promote the seed germination for plantlets production of Brassia biddens, an Amazonian terrestrial orchid by using tissue-culture methods.
In order to evaluate this approach, we performed a factorial experiment with the media containing one of four sucrose concentrations (0 g.L-1, 10 g.L-1, 20 g.L-1 and 30 g.L-1) and one of five concentration of the Knudson and Morels basal medium (control; with full strength; 1/2 ; 1/4 and 1/8 strength). These experiments were performed using a randomized complete block design with ten replicates.
The results were subjected to statistical ANOVA analysis and the differences were tested with Duncans multiple-range test at p ≤ 0.05. The analysis of variance revealed significant differences between treatments with sucrose at 20 g.L-1 and 1/4 strength Knudson and Morels basal medium as the best treatment and there was no interaction between sucrose and the basal medium strength.
The protocol was efficient in promoting the seed germination and has allowed the establishment of in vitro production of orchid plantlets on large scale.
In addition, acclimatized plantlets survived transfer to the greenhouse.
The burning and logging has caused devastation of the Amazon rainforest and loss of species, including orchids.
Tissue culture has proven to be a valuable tool to increase production and preserve orchid species.
Orchids have been multiplied routinely by seeds using a non-symbiotic culture as a propagation method.
The objective of this paper was to promote the seed germination for plantlets production of Brassia biddens, an Amazonian terrestrial orchid by using tissue-culture methods.
In order to evaluate this approach, we performed a factorial experiment with the media containing one of four sucrose concentrations (0 g.L-1, 10 g.L-1, 20 g.L-1 and 30 g.L-1) and one of five concentration of the Knudson and Morels basal medium (control; with full strength; 1/2 ; 1/4 and 1/8 strength). These experiments were performed using a randomized complete block design with ten replicates.
The results were subjected to statistical ANOVA analysis and the differences were tested with Duncans multiple-range test at p ≤ 0.05. The analysis of variance revealed significant differences between treatments with sucrose at 20 g.L-1 and 1/4 strength Knudson and Morels basal medium as the best treatment and there was no interaction between sucrose and the basal medium strength.
The protocol was efficient in promoting the seed germination and has allowed the establishment of in vitro production of orchid plantlets on large scale.
In addition, acclimatized plantlets survived transfer to the greenhouse.
Authors
M. Monteiro do Rêgo, E. Ramalho do Rêgo, H.C. Carvalho dos Santos
Keywords
in vitro culture, genetic resources, Orchidaceae
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