Articles
SEED GERMINATION AND IN VITRO PROPAGATION OF DIANTHUS FRUTICOSUS L.
Article number
813_64
Pages
481 – 484
Language
English
Abstract
Dianthus fruticosus is an endemic plant of Greece found in rocky places of the Cyclades islands Serifos, Sikinos and Folegandros.
It can be used as a bedding plant ideal for rocky gardens and Mediterranean roof gardens.
It can also be a groundcover plant for slope stabilization and restoration of downgraded landscapes in the Mediterranean region.
In this work seed germination conditions were studied and a micropropagation method was developed.
Seeds, surface-sterilized in a 15% commercial bleach water solution for 20 min, were germinated on solid half strength MS medium with 2% sucrose at 15, 20 and 25°C under 16-h photoperiod of 37.5 μmol m-2s-1 fluorescent light.
Germination was 97% at 15°C and was reduced to 47% and 13% at 20 and 25°C, respectively.
Nodal explants from the in vitro germinated seedlings were cultured on solid full strength MS medium with 2% sucrose and various plant growth regulators (NAA, IBA, BA, 2iP, TDZ). In a medium containing 0.1 mg/L NAA combined with 0.5 mg/L 2iP or 0.2 mg/L BA all the explants produced shoots.
The highest multiplication rate was taken in a medium with 0.1 mg/L NAA combined with 0.5 mg/L 2iP and in a medium with 0.1 mg/L IBA combined with 0.1 or 0.5 mg/L BA. In a medium with 2 mg/L IBA all the explants produced simultaneously shoots and roots.
In following subcultures the highest multiplication rate was observed in a medium with 0.1 mg/L NAA combined with 0.5 mg/L 2iP. All shoots produced in various media transplanted on a medium with 2 mg/L IBA rooted very well and plantlets were successfully established ex vitro.
It can be used as a bedding plant ideal for rocky gardens and Mediterranean roof gardens.
It can also be a groundcover plant for slope stabilization and restoration of downgraded landscapes in the Mediterranean region.
In this work seed germination conditions were studied and a micropropagation method was developed.
Seeds, surface-sterilized in a 15% commercial bleach water solution for 20 min, were germinated on solid half strength MS medium with 2% sucrose at 15, 20 and 25°C under 16-h photoperiod of 37.5 μmol m-2s-1 fluorescent light.
Germination was 97% at 15°C and was reduced to 47% and 13% at 20 and 25°C, respectively.
Nodal explants from the in vitro germinated seedlings were cultured on solid full strength MS medium with 2% sucrose and various plant growth regulators (NAA, IBA, BA, 2iP, TDZ). In a medium containing 0.1 mg/L NAA combined with 0.5 mg/L 2iP or 0.2 mg/L BA all the explants produced shoots.
The highest multiplication rate was taken in a medium with 0.1 mg/L NAA combined with 0.5 mg/L 2iP and in a medium with 0.1 mg/L IBA combined with 0.1 or 0.5 mg/L BA. In a medium with 2 mg/L IBA all the explants produced simultaneously shoots and roots.
In following subcultures the highest multiplication rate was observed in a medium with 0.1 mg/L NAA combined with 0.5 mg/L 2iP. All shoots produced in various media transplanted on a medium with 2 mg/L IBA rooted very well and plantlets were successfully established ex vitro.
Authors
M. Papafotiou, J. Stragas
Keywords
acclimatization, micropropagation, native plant, plant growth regulators, rooting, seed germination, shoot multiplication
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