Articles
MOLECULAR AND PHENOTYPICAL CHARACTERIZATION OF TRANSGENIC TOMATO PLANTS: SOLANUM LYCOPERSICON
Article number
823_2
Pages
33 – 40
Language
English
Abstract
The cultivated tomato (Solanum lycopersicon) is one of the major vegetable crops grown throughout the world.
In Tunisia, the improvements in productivity have been modified by many viruses such as TSWV, PVMV or the TYLCV that are now responsible for the decline in tomato production.
In addition the recent emergence of Pepper Vein Mottle Virus (PVMV) in Tunisia is causing further concerns since this virus has never been previously observed in North Africa.
Under these situations there has been an increase in interest in producing PVMV resistant transgenic plants.
An improved protocol for Agrobacterium mediated transformation of 4 tomato cultivars was developed by examining the effects of different factors on the efficiency of transformation and regeneration.
Type of explant (hyp, coty, leaf), explant size, inoculation time, Agrobacterium strain, antibiotics used and frequency of transfer to fresh selective regeneration medium, were found to affect transformation efficiency.
Shoot organogenesis was achieved on media with zeatin (Zea) or indole-3acetic-acid (IAA) combination.
The accessions tested: Riogrande, Justar, Nemador and Agata showed differences in booth their attitude for transformation and regeneration.
Stable integration and transmission of the transgene in R1 generation plants were confirmed by PCR and southern blot.
In Tunisia, the improvements in productivity have been modified by many viruses such as TSWV, PVMV or the TYLCV that are now responsible for the decline in tomato production.
In addition the recent emergence of Pepper Vein Mottle Virus (PVMV) in Tunisia is causing further concerns since this virus has never been previously observed in North Africa.
Under these situations there has been an increase in interest in producing PVMV resistant transgenic plants.
An improved protocol for Agrobacterium mediated transformation of 4 tomato cultivars was developed by examining the effects of different factors on the efficiency of transformation and regeneration.
Type of explant (hyp, coty, leaf), explant size, inoculation time, Agrobacterium strain, antibiotics used and frequency of transfer to fresh selective regeneration medium, were found to affect transformation efficiency.
Shoot organogenesis was achieved on media with zeatin (Zea) or indole-3acetic-acid (IAA) combination.
The accessions tested: Riogrande, Justar, Nemador and Agata showed differences in booth their attitude for transformation and regeneration.
Stable integration and transmission of the transgene in R1 generation plants were confirmed by PCR and southern blot.
Authors
R. Lengliz, F. Gorsane, H. Majoul, H. Fakhfakh
Keywords
Agrobacterium tumefaciens, timentin, transformation, regeneration
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