Articles
CHEMICAL, BIOTECHNOLOGICAL AND PHARMACOLOGICAL APPLICATION OF TOMATO HYBRIDS
Article number
823_4
Pages
49 – 58
Language
English
Abstract
In collaboration with M.F.M. International of Torre del Greco we studied the nutritional quality of several tomato hybrids of Solanum lycopersicum L. Samples were collected from field trials conducted at several sites with different pedoclimatic conditions.
The hydrophilic, lipophilic and methanolic antioxidative activities of these hybrids extracts were determined.
The hydrophilic and lipophilic antioxidant activity was carried out by DMPD (N,N-Dimethyl-p-phenylenediamine dihydrochloride) and ABTS (2,2-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt) methods, respectively.
The acetonic antioxidant activity was performed by the DPPH method and, on this fraction, total polyphenolic content was evaluated by Folin-Ciocalteau assay.
To evaluate potential anti-tumoral activity, cytotoxic activities by brine shrimps assay were also determined in all acetonic extracts.
Moreover the purification of the polysaccharide fraction derived from products and by-products of the tomato industry (Solanum lycopersicum L.) and its chemical composition, rheological properties and partial primary structure on the basis of spectroscopic analyses (UV, IR, 1H NMR) will be reported.
We have investigated the effect of the polysaccharide on nitrite and ROS production in LPS-stimulated J774 macrophages for 24 h.
The tomato peel polysaccharide was inhibited in a concentration-dependent nitrite and ROS production as well as iNOS protein expression induced by LPS. Incubation of cells with the polysaccharide resulted in a significant decrease of NF-kB/DNA binding activity which was correlated with a marked reduction of iNOS mRNA levels.
These results show that polysaccharide inhibits NF-kB activation and iNOS gene expression by preventing the reactive species production, and suggest for this compound a role for controlling oxidative stress and/or inflammation.
The achievements of biodegradable and thermoplastic materials by using chemical and enzymatic processes from polysaccharides are also reported.
The realization of three and more manufactured articles, for example flowerpots or rigid packing vases, film for agriculture use and ensiling film will be in progress.
The hydrophilic, lipophilic and methanolic antioxidative activities of these hybrids extracts were determined.
The hydrophilic and lipophilic antioxidant activity was carried out by DMPD (N,N-Dimethyl-p-phenylenediamine dihydrochloride) and ABTS (2,2-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt) methods, respectively.
The acetonic antioxidant activity was performed by the DPPH method and, on this fraction, total polyphenolic content was evaluated by Folin-Ciocalteau assay.
To evaluate potential anti-tumoral activity, cytotoxic activities by brine shrimps assay were also determined in all acetonic extracts.
Moreover the purification of the polysaccharide fraction derived from products and by-products of the tomato industry (Solanum lycopersicum L.) and its chemical composition, rheological properties and partial primary structure on the basis of spectroscopic analyses (UV, IR, 1H NMR) will be reported.
We have investigated the effect of the polysaccharide on nitrite and ROS production in LPS-stimulated J774 macrophages for 24 h.
The tomato peel polysaccharide was inhibited in a concentration-dependent nitrite and ROS production as well as iNOS protein expression induced by LPS. Incubation of cells with the polysaccharide resulted in a significant decrease of NF-kB/DNA binding activity which was correlated with a marked reduction of iNOS mRNA levels.
These results show that polysaccharide inhibits NF-kB activation and iNOS gene expression by preventing the reactive species production, and suggest for this compound a role for controlling oxidative stress and/or inflammation.
The achievements of biodegradable and thermoplastic materials by using chemical and enzymatic processes from polysaccharides are also reported.
The realization of three and more manufactured articles, for example flowerpots or rigid packing vases, film for agriculture use and ensiling film will be in progress.
Authors
G. Tommonaro, A. Poli, R. De Prisco, B. Nicolaus
Keywords
polyphenolic content, antioxidant activity, NF-κB factor, anti-inflamatory capacity
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