Articles
VIRUS DISEASES SCREENING IN CLONAL SELECTION OF CROATIAN GRAPEVINE CULTIVARS
Article number
827_109
Pages
623 – 626
Language
English
Abstract
There is a large number of grapevine cultivars grown in Croatia, most of which
are considered to be autochthonous.
Unfortunately, clonal selection of these cultivars
has never been done and there is no planting material of adequate quality standards.
As a first step in clonal selection, mass positive selection together with sanitary selection
of 15 economically-important cultivars was initiated.
To this aim, visual observations
were made for the presence of virus-induced symptoms.
Samples of dormant
canes were collected from the vines with positive agronomic traits and no visible
symptoms of virus infection.
The presence of four economically important viruses was
evaluated using ELISA (enzyme linked immunosorbent assay): two nepoviruses,
Grapevine fanleaf virus (GFLV) and Arabis mosaic virus (ArMV) and two closteroviruses
Grapevine leafroll-associated virus 1 (GLRaV-1) and Grapevine leafrollassociated virus 3 (GLRaV-3). Survey of virus diseases in commercial vineyards of
different vine-growing region of Croatia disclosed the widespread presence of viruses
and the low occurrence of non-infected vines.
This is true especially for the coastal
region where it was not possible to find any healthy plant of certain cultivars.
Although no visible symptoms were observed, it was not rare to detect multiple
infections by two or even three viruses.
The most widespread virus in Dalmatia was
GFLaV3 with approximately 80% of vines infected.
The situation is much better in
the northern regions, where almost half of the analyzed plants were healthy and the
most represented virus was GLRaV-1. The presence of both nepoviruses is rather low,
especially ArMV (only 1% of vines infected).
are considered to be autochthonous.
Unfortunately, clonal selection of these cultivars
has never been done and there is no planting material of adequate quality standards.
As a first step in clonal selection, mass positive selection together with sanitary selection
of 15 economically-important cultivars was initiated.
To this aim, visual observations
were made for the presence of virus-induced symptoms.
Samples of dormant
canes were collected from the vines with positive agronomic traits and no visible
symptoms of virus infection.
The presence of four economically important viruses was
evaluated using ELISA (enzyme linked immunosorbent assay): two nepoviruses,
Grapevine fanleaf virus (GFLV) and Arabis mosaic virus (ArMV) and two closteroviruses
Grapevine leafroll-associated virus 1 (GLRaV-1) and Grapevine leafrollassociated virus 3 (GLRaV-3). Survey of virus diseases in commercial vineyards of
different vine-growing region of Croatia disclosed the widespread presence of viruses
and the low occurrence of non-infected vines.
This is true especially for the coastal
region where it was not possible to find any healthy plant of certain cultivars.
Although no visible symptoms were observed, it was not rare to detect multiple
infections by two or even three viruses.
The most widespread virus in Dalmatia was
GFLaV3 with approximately 80% of vines infected.
The situation is much better in
the northern regions, where almost half of the analyzed plants were healthy and the
most represented virus was GLRaV-1. The presence of both nepoviruses is rather low,
especially ArMV (only 1% of vines infected).
Authors
J.K. Kontić, I. Pejić, E. Maletić, B. Sladonja, D. Poljuha, A. Vokurka, G. Zdunić, D. Preiner, S. Šimon, E. Rühl
Keywords
grapevine, clonal selection, virus screening, Grapevine fanleaf virus (GFLV), Arabis mosaic virus (ArMV,) Grapevine leafroll-associated viruses (GLRV 1 and 3)
Online Articles (113)