TRANSFORMATION OF EMBRYOGENIC CALLUS AND TRANSGENIC PLANT REGENERATION IN TABLE GRAPEVINE ‘SUGRAONE’ (VITIS VINIFERA L.): EFFECT OF AGROBACTERIUM TUMEFACIENS STRAIN

Grapevine is one of the major fruit crops in the world.
The use of genetic engineering using Agrobacterium for grapevine improvement appears to be a powerful tool to introduce new traits without any alteration of the essential characters and identity of the cultivar.
Embryogenic calli of ‘Sugraone’ were co-cultured with Agrobacterium tumefaciens strains C58(pMP90) and EHA105 both carrying the binary plasmid pBIN19-sgfp. Transient expression of sgfp gene was scored weekly as number of GFP spots in the calli per Agrobacterium strain.
At 28 days, results were considered as stable expression of sgfp. Although transient expression was higher in calli co-cultured with Agrobacterium strain EHA105, there were no statistical differences at 28 days of infection between the two strains.
Transformation of embryogenic calli resulted in the regeneration of 27 plants from calli co-cultured with EHA (E-plants) and 16 plants from calli co-cultured with PMP (P-plants). Eighteen E-plants and 1 P-plant have been analysed by PCR with sgfp-primers and all were positive.
Southern analyses were performed to confirm the stable insertion of the gene sgfp. Similar experiments have been done with ‘Crimson Seedless’ and GFP positive somatic embryos have been regenerated.

IX International Conference on Grape Genetics and Breeding

A.J. López-Pérez, J. Carreño, M. Dabauza

grapevine, transformation, Agrobacterium

https://doi.org/10.17660/ActaHortic.2009.827.71