Articles
PRODUCTION AND CHARACTERIZATION OF INTERGENERIC HYBRIDS BETWEEN ANEMONE CORONARIA AND RANUNCULUS ASIATICUS
Article number
855_12
Pages
101 – 106
Language
English
Abstract
Anemone coronaria L. and Ranunculus asiaticus L. are common cut flowers which belong to the Ranunculaceae.
Between these species a high degree of variation can be found in leaves, flower morphology and flower colour.
Therefore intergeneric crossings between these species might result in new interesting hybrids.
Crosses between these genera were performed in the greenhouse and pre- and post-zygotic barriers were examined.
Two to five weeks after pollination, immature achenes were harvested and rescued in vitro.
The obtained F1 generation was investigated on morphological, molecular and cytogenetic level.
The F1 plants had very similar flowers to the mother plants and although the limited molecular paternal contribution, AFLP analyses confirmed the hybrid character of the F1 plants.
Chromosome counts showed that the F1 plants were mixoploids, composed of cells with different chromosome numbers.
Between these species a high degree of variation can be found in leaves, flower morphology and flower colour.
Therefore intergeneric crossings between these species might result in new interesting hybrids.
Crosses between these genera were performed in the greenhouse and pre- and post-zygotic barriers were examined.
Two to five weeks after pollination, immature achenes were harvested and rescued in vitro.
The obtained F1 generation was investigated on morphological, molecular and cytogenetic level.
The F1 plants had very similar flowers to the mother plants and although the limited molecular paternal contribution, AFLP analyses confirmed the hybrid character of the F1 plants.
Chromosome counts showed that the F1 plants were mixoploids, composed of cells with different chromosome numbers.
Publication
Authors
E. Dhooghe, D. Reheul, M.-C. Van Labeke
Keywords
distant crosses, intergeneric, wide hybridization, Ranunculaceae
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