Articles
SOMACLONAL VARIATION OF CALADIUM BICOLOR (AIT.) VENT. ‘JAO YING’ AFTER IN VITRO CULTURE PROPAGATION
Article number
855_42
Pages
281 – 288
Language
English
Abstract
In vitro culture of Caladium bicolor by multiple shoot production via callus induction was established.
Explants from the first fully expanded leaf of C. bicolor cv. Jao Ying, a commercial cultivar, were cultured to determine the appropriate concentrations of plant growth regulators in modified Murashige and Skoog medium for rapid micropropagation and variation.
All 16 combinations of N6-benzyladenine (BA) with 1-naphthalene acid (NAA) or 2,4-dichlorophenoxyacetic acid (2,4-D) induced callus.
The combination of 8.88 μM BA with 2.69 μM NAA was the most effective for callus proliferation.
Subsequently, plantlets were regenerated from calli on MS medium containing 11.1 μM BA. Most regenerated plants from in vitro culture, grown in glasshouse conditions, were more vigorous than the original ones.
Within the 10 combinations of BA and NAA, the regenerated plants could be characterized into 13 types.
From each growth regulator combination, 27 types of the regenerated plants were observed.
The occurrence of variants varied from 22.4187.32%. The mode and incidence of variations were discussed.
Many regenerated variants were totally different from the original plants.
Explants from the first fully expanded leaf of C. bicolor cv. Jao Ying, a commercial cultivar, were cultured to determine the appropriate concentrations of plant growth regulators in modified Murashige and Skoog medium for rapid micropropagation and variation.
All 16 combinations of N6-benzyladenine (BA) with 1-naphthalene acid (NAA) or 2,4-dichlorophenoxyacetic acid (2,4-D) induced callus.
The combination of 8.88 μM BA with 2.69 μM NAA was the most effective for callus proliferation.
Subsequently, plantlets were regenerated from calli on MS medium containing 11.1 μM BA. Most regenerated plants from in vitro culture, grown in glasshouse conditions, were more vigorous than the original ones.
Within the 10 combinations of BA and NAA, the regenerated plants could be characterized into 13 types.
From each growth regulator combination, 27 types of the regenerated plants were observed.
The occurrence of variants varied from 22.4187.32%. The mode and incidence of variations were discussed.
Many regenerated variants were totally different from the original plants.
Publication
Authors
A. Thongpukdee, C. Thepsithar, P. Chiensil
Keywords
micropropagation, growth regulators, BA, NAA
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