Articles
IN VITRO SELECTION OF ANTHURIUM ANDREANUM FOR SALT STRESS RESISTANCE
Article number
855_31
Pages
213 – 220
Language
English
Abstract
The youngest leaves, petioles, and roots of micropropagated anthurium explants of cv.
Bolero were regenerated in vitro on media consisting of 1/2 Murashige-Skoog salts with the ammonium nitrate content lowered to 1/4, and with different combinations of growth regulators.
In all the experiments, NaCl was included in the medium at a concentration range of 0, 10, 20, 40, 80, 160 and 320 mM, or 0, 25 and 50 mM. The most effective medium for shoot regeneration contained 0.5 mg/L thidiazuron and 0.5 mg/L NAA. The earliest regeneration was from leaves, then from petioles and roots.
The highest NaCl concentration that the explants could survive and regenerate in was below 40 mM when BAP and 2,4-D were used, and 50 mM when TDZ and NAA were used.
The shoot regeneration was generally preceded by a globular callus that was light green on the medium without NaCl and yellow with scattered green points on the medium with NaCl.
The green shoots on the NaCl medium regenerated 2-3 months later than those on the NaCl-free medium.
Most of these shoots yellowed and proved to be escapers, but five survived prolonged selection.
Bolero were regenerated in vitro on media consisting of 1/2 Murashige-Skoog salts with the ammonium nitrate content lowered to 1/4, and with different combinations of growth regulators.
In all the experiments, NaCl was included in the medium at a concentration range of 0, 10, 20, 40, 80, 160 and 320 mM, or 0, 25 and 50 mM. The most effective medium for shoot regeneration contained 0.5 mg/L thidiazuron and 0.5 mg/L NAA. The earliest regeneration was from leaves, then from petioles and roots.
The highest NaCl concentration that the explants could survive and regenerate in was below 40 mM when BAP and 2,4-D were used, and 50 mM when TDZ and NAA were used.
The shoot regeneration was generally preceded by a globular callus that was light green on the medium without NaCl and yellow with scattered green points on the medium with NaCl.
The green shoots on the NaCl medium regenerated 2-3 months later than those on the NaCl-free medium.
Most of these shoots yellowed and proved to be escapers, but five survived prolonged selection.
Publication
Authors
T. Orlikowska, M. Zawadzka
Keywords
adventitious shoot regeneration, NaCl, salt stress symptoms, TDZ
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