Home > III International Symposium on Longan, Lychee, and other Fruit Trees in Sapindaceae Family > LONG-TERM SUBCULTURE AND MAINTENANCE OF TRANSGENIC RESISTANT EMBRYOGENIC CALLUS IN LITCHI (LITCHI CHINENSIS SONN.)

Articles

LONG-TERM SUBCULTURE AND MAINTENANCE OF TRANSGENIC RESISTANT EMBRYOGENIC CALLUS IN LITCHI (LITCHI CHINENSIS SONN.)

Article number

863_16

Pages

135 – 140

Language

English

Abstract

The effects of subculture method, hygromycin, AgNO₃, inoculation quantity and subculture cycle on the growth of litchi transgenic resistant embryogenic callus (TREC) were studied in this experiment.
Meanwhile, the influences of the medium and long-term subculture on the GUS expression of litchi resistant embryogenic callus were carried out.
The obtained results showed that litchi TREC could be steadily maintained for a long time when they were alternately subcultured in the MS medium containing 1.0 mg/L 2,4-D, 50 mg/L hygromycin and the MS medium supplemented with 1.0 mg/L 2,4-D, 100 mg/L inositol and 50 mg/L hygromycin.

Publication

III International Symposium on Longan, Lychee, and other Fruit Trees in Sapindaceae Family

Authors

C.C. Lai, Z.X. Lai, Q.L. Sang, Z.Y. Huang, Q. Huang

Keywords

litchi (Litchi chinensis Sonn.), transgenosis, resistant embryogenic callus, subculture and maintenance, GUS assay

Full text

https://doi.org/10.17660/ActaHortic.2010.863.16

Online Articles (94)

1. LITCHI PRODUCTION IN THE ASIA-PACIFIC REGION

S.K. Mitra | P.K. Pathak

2. PRODUCTION STATUS OF LONGAN IN CHINA

S.H. Wu

3. MARKETING – THE KEY TO SUCCESS

S. Rasananda

4. SAPINDACEAE PRODUCTION AND RESEARCH IN AUSTRALIA

Y. Diczbalis | B. Nicholls | I. Groves | K. Lake

5. THE BRAZILIAN LYCHEE INDUSTRY – PRESENT AND FUTURE

O.K. Yamanishi | M.C. Pires | L.F.P. Almeida

6. LYCHEE INDUSTRY IN PAKISTAN: POTENTIAL AND PROSPECTS

I.A. Rajwana | A.U. Malik | A.S. Khan | R. Anwar

7. STATUS AND PERSPECTIVES OF FUJIAN LONGAN AND LYCHEE INDUSTRY

Q. Shi | J. Li

8. RESEARCH ON GERMPLASM RESOURCES AND BREEDING OF LONGAN (DIMOCARPUS LONGAN LOUR.) IN THE PAST DECADE IN CHINA

9. CONSTRUCTION OF A HIGH DENSITY MOLECULAR LINKAGE MAP FOR LYCHEE BASED ON AFLP AND RAPD MARKERS

10. MOLECULAR BIOLOGY AND PROTEOMICS DURING SOMATIC EMBRYOGENESIS IN DIMOCARPUS LONGAN LOUR.

11. ANALYSIS OF DONGBI LONGAN WITH ISOENZYME AND RAPD TECHNOLOGIES

D.M. Pan | F.L. Zhong | Z.X. Guo | L. Lin | Z.C. Zheng

12. STUDIES ON IN VITRO GERMPLASM CONSERVATION OF LITCHI

J.F. Wang | Z.Q. Wang

13. CLONING AND SEQUENCE ANALYSIS OF GLYCOLATE OXIDASE HOMOLOGUES GENE (LCGOX2) FROM LITCHI PERICARP

G.B. Hu | Z.Y. Yang | H.C. Wang | Y.H. Qin | R. Ouyang

14. LLFY, A LONGAN LEAFY ORTHOLOG, IS ASSOCIATED WITH DIFFERENTIATION AND MAINTENANCE OF INFLORESCENCE BUD

L.H. Zeng | L. Guan | S.H. Wu

15. THE CREATION AND MOLECULAR IDENTIFICATION OF INTERGENERIC HYBRIDS BETWEEN LITCHI AND LONGAN

16. LONG-TERM SUBCULTURE AND MAINTENANCE OF TRANSGENIC RESISTANT EMBRYOGENIC CALLUS IN LITCHI (LITCHI CHINENSIS SONN.)

C.C. Lai | Z.X. Lai | Q.L. Sang | Z.Y. Huang | Q. Huang

17. CONSTRUCTION OF A MOLECULAR GENETIC LINKAGE MAP FOR LONGAN BASED ON RAPD, ISSR, SRAP AND AFLP MARKERS

18. GENETIC EVALUATIONS OF IN VITRO CONSERVED EMBRYOGENIC CALLI OF LITCHI

Z.Q. Wang | J.F. Wang

19. HIGH FREQUENCY PLANT REGENERATION VIA IN VITRO SOMATIC EMBRYOGENESIS IN FIFTEEN CULTIVARS OF DIMOCARPUS LONGAN LOUR.

X.L. Lin | Z.X. Lai | Q.F. Xu

20. A PRELIMINARY STUDY ON LONGAN ARTIFICIAL CROSSING AND SEVERAL TRAITS OF THE HYBRID POPULATIONS

21. ANALYSES ON ACTIVITIES AND ISOZYMATIC PATTERNS OF SOD DURING EARLY SOMATIC EMBRYOGENESIS IN LONGAN

Y.L. Lin | Z.X. Lai

22. A STUDY ON THE GENETIC EFFECTS ON FRUIT QUALITY TRAITS AND SELECTION OF PROMISING INDIVIDUALS FROM A MAPPING POPULATION OF LITCHI

23. CHANGES OF APX ISOZYMES UNDER HIGH-TEMPERATURE STRESS AND CLONING OF AN APX FUNCTIONAL FRAGMENT IN EMBRYOGENIC CALLUS OF LITCHI

W. Shao | Z.X. Lai | Q. Huang | Y.Q. Guo

24. POD ISOENZYME AND RAPD ANALYSIS ON LITCHI IN FUQING

L.S. Liu | D.M. Pan | F.L. Zhong | J.B. Wang

25. A STUDY ON THE GENETIC EFFECT AND MAS EXPLORATION OF JUVENILE PHASE AND FRUIT MATURATION PERIOD IN A MAPPING POPULATION OF LITCHI

26. IDENTIFICATION OF ‘DONGYUAN’ LONGAN BY RAPD ANALYSIS

Z.B. Zheng | H.J. Luo | S.J. Hu | S. Luo | C.M. Liu

27. THE GERMPLASM RESOURCES AND UTILIZATION OF LONGAN IN QUANZHOU

W.Z. Lin | J.S. Zheng | W.D. Zhuang

28. HORMONAL REGULATION OF THE REGULAR AND ‘OFF SEASON’ FLORAL INDUCTION PROCESS OF LONGAN (DIMOCARPUS LONGAN) TREES

K.F. Bangerth | P. Potchanasin | K. Sringarm

29. ELUCIDATION OF THE FACTORS RESPONSIBLE FOR CRACKING AND SUNBURN IN LITCHI AND INTEGRATED MANAGEMENT TO MINIMIZE THE DISORDERS

S.K. Mitra | P.K. Pathak | S. Debnath | A. Sarkar | D. Mondal

30. RATE AND TIME OF POTASSIUM FERTILIZATION INFLUENCE YIELD AND QUALITY OF LITCHI

P.K. Pathak | S.K. Mitra

31. EVALUATION OF DYNAMIC SUBSTRATES UNDER INTEGRATED PLANT NUTRIENT MANAGEMENT AFFECTING GROWTH YIELD AND QUALITY OF LITCHI (LITCHI CHINENSIS SONN.)

R. Kumar

32. STUDY ON BIOLOGICAL CHARACTERS OF ‘SIJIHUA’ LONGAN

33. STUDY ON THE FLOWERING AND FRUITING PHENOLOGICAL LAW OF ‘SIJIHUA’ LONGAN (DIMOCARPUS LONGAN) AND ITS REGULATION

34. STUDY ON SHRIVELLED-SEED FRUITS IN LONGAN INDUCED BY MH AND 6-BA

M. Li | D.L. Qiu | X.X. Xiao | G.Q. Liu

35. PHYSIOLOGICAL RESPONSES OF LITCHI TO DROUGHT

N. Tang | L.T. Yang | Q. Li | L.S. Chen

36. EFFECT OF TEMPERATURE ON FLOWER-BUD INDUCTION OF LONGAN IN PHYTOTRON

Q.X. Chen | D.M. Pan | L.X. Lu

37. ANALYSIS OF 2-DE PROFILES OF FLORAL REVERSION BUD PROTEINS IN LONGAN

W. Chen | Q. Long | Q.X. Chen | S.Q. Zheng

38. DIFFERENTIAL AND HOUSE-KEEPING PROTEINS OF LONGAN EMBRYOS AT DIFFERENT DEVELOPMENT STAGES

W.Y. Liang | Y. Li | H. Liu | W. Chen

39. A DIFFERENCE IN COLD TOLERANCE AMONG LITCHI CULTIVARS AND ITS MECHANISM

W.Q. She | X.H. Liu

40. HORMONAL CONTROL OF FLOWER INDUCTION IN LITCHI AND LONGAN

41. ANALYSIS OF LONGAN POD ISOENZYME ACTIVITY

F.L. Zhong | Z.X. Guo | T.L. Liu | C.Y. Ma | D.M. Pan

42. EFFECTS OF TEMPERATURE AND POTASSIUM CHLORATE ON LEAF GAS EXCHANGE AND FLOWERING IN LONGAN

43. EFFECTS OF GIRDLING AND DEFOLIATION ON HORMONAL CHANGES DURING FLOWER INDUCTION IN LONGAN (DIMOCARPUS LONGAN LOUR.)

44. THE EFFECT OF FOLIAR SPRAYS OF THE SYNTHETIC AUXIN 3-5-6 TPA, ON FRUIT DROP, FRUIT SIZE AND SEED DEVELOPMENT IN THREE LYCHEE (LITCHI CHINENSIS) CULTIVARS: TAI SO, FAY ZEE SUI AND KWAI MAI PINK

J. Drinnan | Y. Diczbalis

45. THE USE OF HEAT-SUMS TO PREDICT HARVEST DATES FOLLOWING POTASSIUM CHLORATE (KCLO₃) APPLICATION IN LONGAN

Y. Diczbalis | J. Drinnan

46. THE RESPONSE OF LONGAN TREES TO TRAINING SYSTEM AND FERTILIZER MANAGEMENT

C. Senanan | P. Manochai | S. Ussahatanonta | S. Ongprasert

47. LEAF AREA INDEX FOR FLAT SHAPE TRAINED LONGAN (DIMOCARPUS LONGAN LOUR.) TREES TO IMPROVE FRUIT QUALITY AND YIELD

N. Jarassamrit | S. Jeen-on

48. SEASONAL EFFECT OF POTASSIUM CHLORATE ON FLOWERING AND YIELD OF LONGAN (DIMOCARPUS LONGAN LOUR.)

P. Manochai | T. Jaroenkit | S. Ussahatanonta | S. Ongprasert | B. Kativat

49. DEGRADATION AND MOVEMENT OF CHLORATE IN LONGAN PLANTATIONS

S. Ongprasert | W. Wiriya-Alongkorn | W. Spreer

50. THE FACTORS AFFECTING LONGAN FLOWER INDUCTION BY CHLORATE

S. Ongprasert | W. Wiriya-Alongkorn | W. Spreer

51. INFLUENCE OF LONGAN PACKED BED ARRANGEMENT ON AIR FLOW PATTERN UNDER FORCED-AIR CONVECTION

S. Jaturonglumlert | J. Pimphimol | J. Varith

52. MASS TRANSFER KINETICS OF LONGAN LEATHER BETWEEN HOT AIR AND FAR-INFRARED DRYING

S. Jaturonglumlert | T. Kiatsiriroat

53. EFFECT OF FRUIT BAGGING ON CHEMICAL COMPOSITIONS AND SKIN PIGMENTS OF FRESH LONGAN (DIMOCARPUS LONGAN LOUR.)

T. Jaroenkit | S. Maichoo | P. Manochai | S. Ratanamano

54. AEROPONICS TO MONITOR ROOT GROWTH OF LONGAN TREES

W. Wiriya-Alongkorn | S. Ongprasert | W. Spreer | T. Müller | U. Srikasetsarakul

55. THAI TROPICAL LYCHEES – IDENTIFICATION OF CULTIVARS BY MORPHOLOGICAL METHOD

W. Wiriya-Alongkorn | S. Rasananda

56. NUTRITIONAL SURVEY OF LYCHEE ON THE COAST AREA OF THE GULF OF MEXICO, MEXICO

J. Valdivia-Sanchez | A. Trinidad-Santos | R.A. Muñoz-Márquez

57. EVALUATION OF MAXIM^® (3,5,6-TPA) FOR INCREASE IN YIELD, FRUIT SIZE AND RETENTION IN LITCHI, CV. HLH MAURITIUS, IN SOUTH AFRICA

R.B. Cronje | P.G. Mostert

58. A HOLISTIC APPROACH TO IMPROVE LITCHI ORCHARD MANAGEMENT IN SOUTH AFRICA

R.B. Cronje | P.G. Mostert

59. SEASONAL CHANGES IN STARCH CONCENTRATION IN RELATION TO TREE PHENOLOGY, ORCHARD MANAGEMENT AND ALTERNATE BEARING IN LITCHI (LITCHI CHINENSIS SONN.) IN SOUTH AFRICA

R.B. Cronje | P.G. Mostert

60. DETERIORATION OF ANTHOCYANINS IN LITCHI ‘KOM’ FRUIT STORED UNDER DIFFERENT RELATIVE HUMIDITY LEVELS

N. Somboonkaew | L.A. Terry

61. SHOOT GROWTH FROM SCIONS TOP-GRAFTED ON DIFFERENT LITCHI CULTIVARS

G.B. Hu | H.B. Chen | X.M. Huang | J.H. Bu

62. CHANGES IN ANTIOXIDANT ENZYME ACTIVITIES AND HYDROGEN PEROXIDE CONCENTRATION IN LITCHI CHINENSIS DURING FLORAL DIFFERENTIATION

63. PHYSIOLOGICAL COMPARISON OF ABSCISING RUDIMENTARY LEAF AND VIGOROUSLY DEVELOPING PANICLE IN LITCHI: ANTIOXIDANT ENZYMES AND PHYTOHORMONES

64. A STUDY ON THE NPK NUTRITION IN AUTUMN SHOOTS OF ‘FEIZIXIAO’ LITCHI

J. Chen | G.M. Sun | J.H. Xie | X.L. Fan

65. AN OVERVIEW OF FACTORS RELATED TO FRUIT SIZE IN LITCHI CHINENSIS SONN.

J.G. Li | X.M. Huang | H.B. Huang

66. STUDY OF FLOWERING AND FRUITING CHARACTERISTICS OF TWO STRAINS OF EARLY SEASON LONGAN (DIMOCARPUS LONGAN LOUR.)

J.C. Pan | G.X. Huang | L.J. Liu | F. Li

67. SELECTION AND BREEDING OF ‘GUINUO’ (LITCHI CHINENSIS SONN.) – A NEW CULTIVAR OF BIG FRUIT SIZE

68. RELATIONSHIP BETWEEN FLORAL BUD INDUCTION BY APPLICATION OF KCLO₃ AND NITRATE REDUCTASE ACTIVITY OF LEAVES ON LONGAN

Z.P. Tang | Y.L. Chen | H.Y. Den | J.C. Pan | C.J. Wang

69. PRELIMINARY REPORT ON ENVIRONMENTAL QUALITY EVALUATION OF PRODUCTION AREA FOR POLLUTION-FREE LONGAN (DIMOCARPUS LOUR.) IN CHONGQING

Y.G. Zhang | Z.Y. Huang | L.H. Li | L. Hong | X.Q. Zhou

70. MAIN PROBLEMS AND COUNTERMEASURES FOR LONGAN POSTHARVEST MANAGEMENT IN CHINA

H.T. Lin | Y.H. Chen | Y.F. Lin

71. CHANGES OF PROTEIN COMPOSITIONS IN POSTHARVEST LONGAN FRUIT

Z.Q. Lin | Z.X. Guo | L. Lin | F.L. Zhong | D.M. Pan

72. STANDARDIZATION OF HANDLING TECHNIQUES OF LITCHI

I. Chakraborty | J. Joseph | A.K. Chaurasiya

73. THERMAL KINETIC STUDY OF COLOR CHANGES AND 5-HYDROXYMETHYL FURFURAL FORMATION IN LITCHI JUICE

74. ALLEVIATION OF CHILLING INJURY IN LITCHI FRUIT BY ABA APPLICATION

W.R. Hu | S.Z. Liu | X.Q. Pang | Z.L. Ji | Z.Q. Zhang

75. RELATIONSHIP BETWEEN THE CHANGES OF PERICARP MICROSTRUCTURE AND FRUIT DECAY OF LONGAN FRUIT DURING STORAGE

S.Z. Guo | H.J. Gao | Q.K. Ma | D.L. Qiu

76. PURIFICATION AND CHARACTERIZATION OF ANTHOCYANIN DEGRADATION ENZYME FROM LITCHI FRUIT PERICARP

77. ICE-TEMPERATURE STORAGE COMBINED WITH STAGE-WISE SO₂ RELEASER TREATMENT EXTENDED SHELF LIFE OF LONGAN FRUIT

H.L. Zhang | Z.Q. Zhang | W.R. Hu | J.J. Gong | X.Q. Pang

78. STUDY ON THE ROLE OF CASPASE-3 AT LONGAN PULP AUTOLYSIS

Z.X. Wu | Z.J. Chen | H.Y. Zhang | D.M. Han | J.G. Li

79. ANTIOXIDANT CAPACITY AND PHENOLIC COMPOUNDS IN LITCHI (LITCHI CHINENSIS SONN.) PERICARP

Z.Q. Hu | X.M. Huang | H.B. Chen | H.C. Wang

80. EFFECT OF ULTRA-HIGH PRESSURE ON THE QUALITY AND SAFETY OF FRESH-CUT LONGAN FRUIT

G.Y. Zhao | B. Li

81. STUDY ON THE ULTRA-HIGH PRESSURE EXTRACTION OF POLYSACCHARIDES FROM LITCHI FRUIT

W. Zong | H.J. Ma

82. FRUIT WEIGHT LOSS AND PERICARP WATER LOSS OF HARVESTED LONGAN FRUIT IN RELATION TO PERICARP BROWNING

H.T. Lin | L. Chen | Y.F. Lin | Y.M. Jiang

83. QUALITY CHANGES OF FRESH LONGAN (DIMOCARPUS LONGAN) UNDER DIFFERENT STORAGE CONDITIONS

T. Fang | J.Q. Chen | H. Peppelenbos

84. APPLICATION OF PEF AND FREEZE CONCENTRATION PROCESSING TECHNOLOGY ON LITCHI JUICE

T. Fang | J.Q. Chen

85. LATE MATURING LONGAN PERICARP COLOR AFFECTED BY THE CHANGES OF PIGMENT AND TOTAL PHENOLS CONTENTS IN THE PERIOD OF FRUIT KEEPING FRESH ON PLANT

86. ENZYMATIC BROWNING OF POSTHARVEST LITCHI: A REVIEW

J.B. Wang | X.S. Wang | Z.Q. Jin

87. MANAGEMENT OF INSECT PESTS AND DISEASES OF LITCHI AND LONGAN IN CHINA

88. PRELIMINARY STUDY ON THE LEAF-GALL MIDGE ASPHONDYLIA SP. – A NEW INSECT PEST OF LONGAN (SAPINDACEAE) IN CHINA

89. CONTROL EFFECTS ON LITCHI DOWNY BLIGHT DISEASE BY ENDOPHYTIC BACTERIAL STRAIN TB2 AND ITS PATHOGENESIS-RELATED PROTEINS

X.Q. Cai | N. Lin | W. Chen | F.P. Hu

90. LITCHI DELICACIES – FEW VALUE ADDED ITEMS PAR EXCELLENCE

I. Chakraborty | A.K. Chaurasiya | J. Saha

91. LITCHI GOOD FOR HEATH FROM SKIN TO HEART: AN OVERVIEW OF LITCHI FUNCTIONAL ACTIVITIES AND COMPOUNDS

92. THE PRELIMINARY DETERMINATION OF VOLATILE CONSTITUENTS OF LONGAN ARILLUS, EPICARPIUM AND SEEDS BY GAS CHROMATOGRAPHY-MASS SPECTROMETRY COUPLED WITH SOLID-PHASE MICROEXTRACTION

93. SIMULATION ON LONGAN TREE MORPHOLOGY AND GROWTH BASED ON L-SYSTEMS

R.J. Wu | C.C. Chen | L.Y. Tang | H.B. Ma

94. DETERMINATION OF GALLIC ACID IN LONGAN FRUITS BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

C.L. Ma | T. Hu | F.H. Ma