Articles
THE FIRST REPORT ON IN VITRO CULTURE OF MUSK ROSE
Article number
870_28
Pages
213 – 218
Language
English
Abstract
Musk rose is an important rose species for extracting rose water and rose oil, which is originated from Iran.
This species has also uses in medical and food industries.
Traditional methods of propagation of Musk rose (e.g. sucker, cutting, budding and grafting) are very slow and time-consuming and are usually associated with various problems such as limitation of stock plants and a prolonged production time.
There is no report on in vitro propagation of Musk rose.
This study was conducted on Rosa moschata to study the factors affecting micropropagation of this species for the first time.
Results showed that, among the various concentrations of MS medium (MS, 1/2 MS, 1/3 MS, 1/4 MS), 1/2 MS medium was the best medium for proliferation.
Comparing TDZ, BAP and KIN showed that TDZ was the best growth regulator for shoot proliferation.
The highest percentage of proliferation was observed with the concentration of 1.25 mg/L TDZ. Using 1/2 MS + 1.5 mg/L TDZ + 0.1 mg/L NAA resulted in longest shoot (15 mm) in proliferation stage.
The best shoot multiplication rate was observed in first subculture (4.71 shoot/explant) and thereafter this trait was decreased.
The best treatment for rooting of microcuttings was obtained with 0.1 mg/L IBA + 0.1 mg/L 2,4-D. Plantlets were acclimatized in a medium consisting of 1:1 (v/v) peat moss and perlite and plantlets were successfully transferred to the greenhouse after 25 days.
This species has also uses in medical and food industries.
Traditional methods of propagation of Musk rose (e.g. sucker, cutting, budding and grafting) are very slow and time-consuming and are usually associated with various problems such as limitation of stock plants and a prolonged production time.
There is no report on in vitro propagation of Musk rose.
This study was conducted on Rosa moschata to study the factors affecting micropropagation of this species for the first time.
Results showed that, among the various concentrations of MS medium (MS, 1/2 MS, 1/3 MS, 1/4 MS), 1/2 MS medium was the best medium for proliferation.
Comparing TDZ, BAP and KIN showed that TDZ was the best growth regulator for shoot proliferation.
The highest percentage of proliferation was observed with the concentration of 1.25 mg/L TDZ. Using 1/2 MS + 1.5 mg/L TDZ + 0.1 mg/L NAA resulted in longest shoot (15 mm) in proliferation stage.
The best shoot multiplication rate was observed in first subculture (4.71 shoot/explant) and thereafter this trait was decreased.
The best treatment for rooting of microcuttings was obtained with 0.1 mg/L IBA + 0.1 mg/L 2,4-D. Plantlets were acclimatized in a medium consisting of 1:1 (v/v) peat moss and perlite and plantlets were successfully transferred to the greenhouse after 25 days.
Authors
M. Khosh-Khui, M. Honarvar, K. Javidnia
Keywords
micropropagation, plant growth regulators, Rosa moschata, TDZ
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