Articles
DISTRIBUTION AND ACCUMULATION OF FLORAL SCENT COMPOUNDS IN ROSE FLOWERS, AND THE CHANGE IN THE GENE EXPRESSION INVOLVED IN THE BIOSYNTHESIS OF THESE COMPOUNDS DURING THE UNFURLING PROCESS
Article number
870_31
Pages
235 – 240
Language
English
Abstract
The concentrations of the aroma components 2-phenylethanol (2PE) and 3,5-dimethoxytoluene (DMT) emitted from the flower of Rosa Yves Piaget change remarkably during the unfurling process.
At each unfurling stage, flowers were separated into the following parts: outer petals, middle petals, and inner petals.
The floral scent components were extracted from each sample and subjected to GC-MS analysis. 2PE accumulated mostly at the initial unfurling stage in outer petals, and DMT in inner petals.
In addition we examined the gene expression levels corresponding to the enzymes involved in the biosyntheses of DMT by real time RT-PCR.
At each unfurling stage, flowers were separated into the following parts: outer petals, middle petals, and inner petals.
The floral scent components were extracted from each sample and subjected to GC-MS analysis. 2PE accumulated mostly at the initial unfurling stage in outer petals, and DMT in inner petals.
In addition we examined the gene expression levels corresponding to the enzymes involved in the biosyntheses of DMT by real time RT-PCR.
Authors
X. Chen, H. Kobayashi, Y. Suzuki, M. Sakai, T. Shimeno, K. Yamaguchi, N. Watanabe, H. Doura
Keywords
2-phenylethanol, 3,5-dimethoxytoluene, Rosa ‘Yves Piaget’, GC-MS, real time RT-PCR, gene expression level
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