Articles
IN VITRO PROPAGATION OF CALLISTEMON CITRINUS
Article number
885_36
Pages
267 – 270
Language
English
Abstract
Callistemon citrinus is a shrub with high ornamental value.
It is highly recommended for use in the Urban Landscape as it is a pollution tolerant plant.
Commercially it is propagated by shoot tip cuttings.
Target of present work was the development a micropropagation protocol.
In vitro cultures were initiated at higher percentages from one-node explants excised from the basal part of young softwood shoots of mature plants end of May, which were cultured on solid (8 g L-1 agar) Woody Plant Medium (WPM) salts, with 100 mg L-1 myo-inositol, 1 mg L-1 thiamine, 0.5 mg L-1 pyridoxine, 0.5 mg L-1 nicotinic acid and 30 g L-1 sucrose, supplemented with 1 mg L-1 BA or 1 mg L-1 NAA and 0.25 mg L-1 BA. High in vitro shoot proliferation was taken when one-node explants excised from microshoots were subcultured in media with 0.25 mg L-1 BA or with 0.5 mg L-1 BA and 0.25 mg L-1 NAA. One-node explants subcultured in media with only NAA gave one shoot and many roots.
All microshoots rooted when transferred in a medium with 0.5 mg L-1 NAA, while 0.5 mg L-1 IBA induced lower rooting percentage (63%); the number of roots produced was also less in the IBA-medium.
All plantlets were successfully acclimatized ex vitro in peat:perlite (1:1 v/v).
It is highly recommended for use in the Urban Landscape as it is a pollution tolerant plant.
Commercially it is propagated by shoot tip cuttings.
Target of present work was the development a micropropagation protocol.
In vitro cultures were initiated at higher percentages from one-node explants excised from the basal part of young softwood shoots of mature plants end of May, which were cultured on solid (8 g L-1 agar) Woody Plant Medium (WPM) salts, with 100 mg L-1 myo-inositol, 1 mg L-1 thiamine, 0.5 mg L-1 pyridoxine, 0.5 mg L-1 nicotinic acid and 30 g L-1 sucrose, supplemented with 1 mg L-1 BA or 1 mg L-1 NAA and 0.25 mg L-1 BA. High in vitro shoot proliferation was taken when one-node explants excised from microshoots were subcultured in media with 0.25 mg L-1 BA or with 0.5 mg L-1 BA and 0.25 mg L-1 NAA. One-node explants subcultured in media with only NAA gave one shoot and many roots.
All microshoots rooted when transferred in a medium with 0.5 mg L-1 NAA, while 0.5 mg L-1 IBA induced lower rooting percentage (63%); the number of roots produced was also less in the IBA-medium.
All plantlets were successfully acclimatized ex vitro in peat:perlite (1:1 v/v).
Authors
M. Papafotiou, A. Skylourakis
Keywords
micropropagation, Myrtaceae, plant growth regulators, rooting, shoot multiplication, tissue culture, woody ornamental, ornamental shrub
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