APPLICATION OF GISH TO CHARACTERISE BUDDLEJA AND HIBISCUS HYBRIDS

Genomic In Situ Hybridization (GISH) is a powerful technique for detecting parental origin of interspecific hybrids and monitoring alien chromatin introgressed in breeding lines.
In this study, successful use of GISH for woody ornamentals, characterized by a small genome and high numbers of small-sized chromosomes, is demonstrated.
GISH was performed on Buddleja and Hibiscus hybrids resulting from an interspecific breeding program between different species of both genera.
Using B. globosa as a probe, GISH analyses on Buddleja ×weyeriana (a F₂ of B. globosa × B. davidii) and F₁ and F₂ hybrids of B. davidii × B. ×weyeriana crosses proved that all chromatin material of B. globosa was introgressed into the B. davidii chromosomes.
Also F₂ hybrids of H. syriacus × H. paramutabilis were analysed using GISH with H. syriacus as a probe.
Also here recombinant chromosomes showing introgression of H. syriacus DNA in H. paramutabilis were detected.

I International Symposium on Woody Ornamentals of the Temperate Zone

K. Van Laere, L. Khrustaleva, J. Van Huylenbroeck, E. Van Bockstaele

Buddleja, Hibiscus, interspecific hybridization, genomic in situ hybridization

https://doi.org/10.17660/ActaHortic.2010.885.57