Articles
IN VITRO REGENERATION OF RHODODENDRONS VIA AXILLARY SHOOT AND SOMATIC EMBRYO INDUCTION
Article number
885_58
Pages
417 – 423
Language
English
Abstract
Efficient in vitro regeneration via axillary shoot induction from shoot-tip meristems and somatic embryo formation from leaf explants were studied with six cultivars of Rhododendron L. The cultivars tested significantly showed the highest shoot regeneration on MS medium containing 6 mg/L 2iP. For most of the cultivars a significantly enhanced shoot multiplication in higher isopentenyladenine (2iP) concentrations (8-10 mg/L) combined with 1 mg/L indole-3-acetic acid (IAA) was found.
Chromosome diploid number (2n=2x=26) in actively growing shoot apices and a high degree of genetic stability were determined.
At concentration 2.5 mg/L thidiazuron (TDZ), the percentage of explants forming embryogenic structures was 70-73%; whereas at concentration of 5 mg/L TDZ, the percentage significantly decreased to 58%. In rhododendrons, somatic embryo induction was observed in the presence of leaf bud formation.
Histological analysis demonstrated embryogenic capacity of callus exclusively in the presence of TDZ.
Chromosome diploid number (2n=2x=26) in actively growing shoot apices and a high degree of genetic stability were determined.
At concentration 2.5 mg/L thidiazuron (TDZ), the percentage of explants forming embryogenic structures was 70-73%; whereas at concentration of 5 mg/L TDZ, the percentage significantly decreased to 58%. In rhododendrons, somatic embryo induction was observed in the presence of leaf bud formation.
Histological analysis demonstrated embryogenic capacity of callus exclusively in the presence of TDZ.
Authors
H. Vejsadová
Keywords
Rhododendron L., shoot-tip meristem, cytology, leaf tissue, morphogenesis, histology
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