Articles
DETERMINATION OF RESISTANCE TO SHARKA (PLUM POX) IN SOME ROMANIAN APRICOT PROGENIES AND ROOTSTOCKS
Article number
899_15
Pages
117 – 122
Language
English
Abstract
Sharka, caused by Plum pox virus (PPV), is the most devastating viral disease affecting stone fruit crops in Europe.
It causes important economic losses in the fruit production, mainly in apricot and European plum.
The spreading of PPV might be limited by planting PPV resistant or at least less-susceptible rootstocks on which PPV resistant scions have been grafted.
In spring 2008, at USAMV Bucharest, Romania, a breeding program aiming to develop cultivars and rootstocks resistant to PPV was initiated and an efficient procedure for the determination of sharka resistance within the progenies was established.
It is based on the methodology developed at IVIA Valencia, Spain.
Peach GF305 rootstocks were used as indicators for susceptibility to PPV in comparison with the Myrobolan BN 4 Kr considered as resistant to sharka (Minoiu et al., 2002; Zagrai et al., 2009). The subsequent grafting protocol was optimized, and a Romanian PPV-D isolate was identified and used as inoculums source.
The two rootstocks were grafted with apricot individuals originating from crossing between a PPV resistant genitor (e.g., SEO, NJA2) and Romanian preferred cultivars.
Grafted plants were inoculated by chip-budding and monitored by visual inspection and ELISA, completed by IC/RT-PCR for the PPV negative plants.
Preliminary data of the Romanian PPV resistance breeding programs will be presented.
It causes important economic losses in the fruit production, mainly in apricot and European plum.
The spreading of PPV might be limited by planting PPV resistant or at least less-susceptible rootstocks on which PPV resistant scions have been grafted.
In spring 2008, at USAMV Bucharest, Romania, a breeding program aiming to develop cultivars and rootstocks resistant to PPV was initiated and an efficient procedure for the determination of sharka resistance within the progenies was established.
It is based on the methodology developed at IVIA Valencia, Spain.
Peach GF305 rootstocks were used as indicators for susceptibility to PPV in comparison with the Myrobolan BN 4 Kr considered as resistant to sharka (Minoiu et al., 2002; Zagrai et al., 2009). The subsequent grafting protocol was optimized, and a Romanian PPV-D isolate was identified and used as inoculums source.
The two rootstocks were grafted with apricot individuals originating from crossing between a PPV resistant genitor (e.g., SEO, NJA2) and Romanian preferred cultivars.
Grafted plants were inoculated by chip-budding and monitored by visual inspection and ELISA, completed by IC/RT-PCR for the PPV negative plants.
Preliminary data of the Romanian PPV resistance breeding programs will be presented.
Publication
Authors
L. Ion, D. Hoza, C. Moale, A.M. Petrica, T. Neagu, I. Zagrai, M. Isac, S. Preda
Keywords
Plum pox virus, apricot, cultivars, rootstocks, resistance
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