Articles
INHERITANCE OF SILENCING IN TRANSGENIC PLUMS
Article number
899_18
Pages
139 – 144
Language
English
Abstract
We have studied the heritability of the virus transgene engineered in HoneySweet plum through different cross-hybridization with two commercial cultivars of Prunus domestica (Prunier dEnte 303 and Quetsche 2906) and one wild species, P. spinosa 2862 rootstock using HoneySweet plum as the pollen donor.
Over all crosses 46% of the progeny were transgenic.
Chi square analyses indicated that the transgene was inherited as a single dominant allele.
Transgenic hybrids were challenged with Plum pox virus (PPV) and resistance was analyzed through more than three dormancy cycles.
Transgenic hybrids were highly resistant to sharka disease while the untransformed sibling hybrids were susceptible to PPV. As in the HoneySweet parent, silencing was shown to be the mechanism of resistance.
These results confirmed the high potential of HoneySweet plum for PPV resistance breeding programmes.
Over all crosses 46% of the progeny were transgenic.
Chi square analyses indicated that the transgene was inherited as a single dominant allele.
Transgenic hybrids were challenged with Plum pox virus (PPV) and resistance was analyzed through more than three dormancy cycles.
Transgenic hybrids were highly resistant to sharka disease while the untransformed sibling hybrids were susceptible to PPV. As in the HoneySweet parent, silencing was shown to be the mechanism of resistance.
These results confirmed the high potential of HoneySweet plum for PPV resistance breeding programmes.
Publication
Authors
M. Ravelonandro, R. Scorza, P. Briard, B. Lafargue, R. Renaud
Keywords
silencing, transgenic, Prunus domestica, P. spinosa, Plum pox virus
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