Articles
ALMOND (PRUNUS DULCIS) AS A HOST OF PLUM POX VIRUS
Article number
899_8
Pages
73 – 78
Language
English
Abstract
The interest in almond (Prunus dulcis) growing increased in Bulgaria during the first decade of the 21st century.
This circumstance dictated a sustained monitoring directed to the immune reaction of cultivars, planted in new orchards, to the important viruses.
The investigation was realized during 20072010 and it aimed to check the susceptibility of the species P. dulcis to Plum pox virus (PPV).
The research was focused on the susceptibility of almond shoots grafted in the crown of plum tree (Prunus domestica) infected by PPV. This tree had a role of a permanent infectious donor for the almond shoots developed after the grafting.
The symptoms of plum pox were well manifested on the leaves of the plum tree.
PPV in the infectious donor was confirmed by routine double antibody sandwich (DAS)-ELISA. In the same time the leaves of almond shoots remain without symptoms of Plum pox and the results obtained by DAS-ELISA were negative.
PPV was detected in the same almond sample by means of immunocapture reverse-transcription polymerase chain reaction (IC-RT-PCR). PPV was not found in the leaf samples taken from the almond mother tree grown in the same area nearby PPV-infected plum trees.
This circumstance dictated a sustained monitoring directed to the immune reaction of cultivars, planted in new orchards, to the important viruses.
The investigation was realized during 20072010 and it aimed to check the susceptibility of the species P. dulcis to Plum pox virus (PPV).
The research was focused on the susceptibility of almond shoots grafted in the crown of plum tree (Prunus domestica) infected by PPV. This tree had a role of a permanent infectious donor for the almond shoots developed after the grafting.
The symptoms of plum pox were well manifested on the leaves of the plum tree.
PPV in the infectious donor was confirmed by routine double antibody sandwich (DAS)-ELISA. In the same time the leaves of almond shoots remain without symptoms of Plum pox and the results obtained by DAS-ELISA were negative.
PPV was detected in the same almond sample by means of immunocapture reverse-transcription polymerase chain reaction (IC-RT-PCR). PPV was not found in the leaf samples taken from the almond mother tree grown in the same area nearby PPV-infected plum trees.
Publication
Authors
N. Petrov, A. Stoev
Keywords
Plum pox virus, almond, IC-RT-PCR
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