Articles
INDUCTION OF SOMATIC EMBRYOGENESIS OF THREE DIFFERENT MANGO (MANGIFERA INDICA L.) GENOTYPES
Article number
992_37
Pages
283 – 288
Language
English
Abstract
Somatic embryogenesis is useful for preservation, clonal multiplication and genetic manipulation.
The present study established a somatic embryogenesis system of three mango genotypes: Baixiangya, a polyembryonic cultivar; Tainong 1, a monoembryonic cultivar and Guifei. Immature fruits were collected from these cultivars 40-60 days after anthesis in the Germplasm Research Institute of Tropical Crops of the Chinese Academy of Tropical Agriculture Sciences, Hainan, China during February and March.
The ovules were aseptically removed from the immature seeds and cultured on the modified B5 medium with 2.74 mM L-glutamine, 0.57 mM ascorbic acid, 6% (w/v) sucrose and 0.8% (w/v) agar powder.
Induction of embryogenic competence was controlled by genotype and various growth regulators (2,4D or 6-BA alone, or 2,4D with 6-BA). Explants grown on the basal medium (control) were also inductive but at a very low efficiency (<10%). All treatments were significantly inductive compared with the control. Baixiangya cultivar had the highest embryo¬genic potential, followed by Guifei and Tainong 1. The most effective culture was Baixiangya cultivar treated with 2,4-D 5 mg/l + 6-BA 2 mg/l.
The present study established a somatic embryogenesis system of three mango genotypes: Baixiangya, a polyembryonic cultivar; Tainong 1, a monoembryonic cultivar and Guifei. Immature fruits were collected from these cultivars 40-60 days after anthesis in the Germplasm Research Institute of Tropical Crops of the Chinese Academy of Tropical Agriculture Sciences, Hainan, China during February and March.
The ovules were aseptically removed from the immature seeds and cultured on the modified B5 medium with 2.74 mM L-glutamine, 0.57 mM ascorbic acid, 6% (w/v) sucrose and 0.8% (w/v) agar powder.
Induction of embryogenic competence was controlled by genotype and various growth regulators (2,4D or 6-BA alone, or 2,4D with 6-BA). Explants grown on the basal medium (control) were also inductive but at a very low efficiency (<10%). All treatments were significantly inductive compared with the control. Baixiangya cultivar had the highest embryo¬genic potential, followed by Guifei and Tainong 1. The most effective culture was Baixiangya cultivar treated with 2,4-D 5 mg/l + 6-BA 2 mg/l.
Publication
Authors
Junya Wei, Yeyuan Chen, Yueyang Zhang, Aiping Gao, Debing Liu
Keywords
mango, somatic embryogenesis, induced
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