Articles
INTERGENERIC HYBRIDIZATION BETWEEN BRASSICA OLERACEA AND ERUCA SATIVA
Article number
1005_7
Pages
97 – 103
Language
English
Abstract
Intergeneric sexual hybrids have been generated between Brassica oleracea (2n = 18, CC) and Eruca sativa (2n = 22, EE). Aim of the project was the combination of the Eruca sativa nuclear genome with the CMS inducing Ogura-cytoplasm of a cauliflower breeding line.
The F1 plants obtained showed an intermediary phenotype, an almost normal growth habit, expressed male sterility, but no seed set after pollination with the Eruca parent.
Chromosome doubling of F1 plants by colchicine treatment resulted in five allotetraploid plants (CCEE). Backcrosses with the E. sativa parent supported by embryo rescue resulted in BC1 plants, which resembled more or less the Eruca phenotype and were completely male sterile.
Repeated backcrosses with E. sativa pollen resulted in BC2 and BC3 progenies.
The hybrid status of the F1 and the elimination of B. oleracea chromosomes were checked by RAPD-PCR, flow cytometry and GISH analysis.
Mitochondrial DNA was tested by Southern hybridization confirming maternal inheritance.
Flower-biological and flower-histological investigations were carried out for each backcross progeny.
BC3 plants, containing exactly the expected 2n = 22 chromosomes of E. sativa, expressed a highly vigorous phenotype and stable male sterility as prerequisite for successful application in Eruca hybrid breeding.
The F1 plants obtained showed an intermediary phenotype, an almost normal growth habit, expressed male sterility, but no seed set after pollination with the Eruca parent.
Chromosome doubling of F1 plants by colchicine treatment resulted in five allotetraploid plants (CCEE). Backcrosses with the E. sativa parent supported by embryo rescue resulted in BC1 plants, which resembled more or less the Eruca phenotype and were completely male sterile.
Repeated backcrosses with E. sativa pollen resulted in BC2 and BC3 progenies.
The hybrid status of the F1 and the elimination of B. oleracea chromosomes were checked by RAPD-PCR, flow cytometry and GISH analysis.
Mitochondrial DNA was tested by Southern hybridization confirming maternal inheritance.
Flower-biological and flower-histological investigations were carried out for each backcross progeny.
BC3 plants, containing exactly the expected 2n = 22 chromosomes of E. sativa, expressed a highly vigorous phenotype and stable male sterility as prerequisite for successful application in Eruca hybrid breeding.
Authors
T. Nothnagel, H. Budahn, O. Schrader , E. Klocke
Keywords
cytoplasmic male sterility, flow cytometry, GISH, molecular markers
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