Articles
INCREASE OF PLANT RESISTANCE TO DISEASES, PESTS AND STRESSES WITH NEW BIOSTIMULANTS
Article number
1009_27
Pages
225 – 233
Language
English
Abstract
In the greenhouse experiments the antipathogenic activity of new polycomponent plant growth regulators (РGRs) Regoplant, Stimpo and Radostim have been investigated by cultivating different cultivars of winter wheat, soy and corn plants on infectious backgrounds.
The best biological efficiency against phytopathogens was obtained by preseeding treatments of seeds and spraying crops in vegetation period with Regoplant (up to 98%) and Stimpo (up to 89%), while Radostim showed up to 74% bioprotective efficiency compared to the control (without treatment by PGRs). By the second generation of wheat and chickpea plants (infected by pathogenic micromycetes of Fusarium L. genus without treatment by РGRs), there was increased resistance to pathogenic micromycetes of Fusarium L. genus.
Using the DOT-blot hybridization method, the considerable difference between mRNA of control wheat and chickpea seedlings and small regulatory si/miRNA of experimental seedlings (obtained from seeds of the first generation of plants, infected by pathogenic micromycetes of Fusarium L. genus and treated by PGRs) was found.
It is proposed that the indicated difference is connected with partial reprograming of the plant cells genome under the impact of PGRs – inductors of synthesis si/miRNA with antipathogenic properties.
The best biological efficiency against phytopathogens was obtained by preseeding treatments of seeds and spraying crops in vegetation period with Regoplant (up to 98%) and Stimpo (up to 89%), while Radostim showed up to 74% bioprotective efficiency compared to the control (without treatment by PGRs). By the second generation of wheat and chickpea plants (infected by pathogenic micromycetes of Fusarium L. genus without treatment by РGRs), there was increased resistance to pathogenic micromycetes of Fusarium L. genus.
Using the DOT-blot hybridization method, the considerable difference between mRNA of control wheat and chickpea seedlings and small regulatory si/miRNA of experimental seedlings (obtained from seeds of the first generation of plants, infected by pathogenic micromycetes of Fusarium L. genus and treated by PGRs) was found.
It is proposed that the indicated difference is connected with partial reprograming of the plant cells genome under the impact of PGRs – inductors of synthesis si/miRNA with antipathogenic properties.
Authors
S.P. Роnоmаrenko, Z.M. Hrytsaenko, V.A. Tsygankova
Keywords
pathogenic and parasitic organisms, growth regulators, DOT-blot hybridization, si/miRNA, heterosis-like effect
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