Articles
BIOLISTIC TRANSFER OF HOP VIROID DISEASE SYNDROME FROM SLOVENIAN CULTIVAR ‘CELEIA’ TO CZECH HOP ‘OSVALD’S 72’: PATHOGENESIS SYMPTOMS AND IDENTIFICATION OF DOMINANT SEQUENCE UPON TRANSFER OF HPSVD COMPONENT
Article number
1010_14
Pages
121 – 127
Language
English
Abstract
A viroid disease syndrome that was recently described on Slovenian cultivar Celeia was investigated.
The disease was transferred using biolistic approach to Czech hop under isolated experimental conditions.
A complex of strong symptoms appeared on cultivar Osvalds 72 that was quite comparable to cultivar Celeia; low plant fitness strong stunting, leaf malformation, small (tiny) leaves and frequently visible leaf mosaic, which was not associated with virus infection. Hop stunt viroid (HpSVd) was analyzed in detail in the inoculated Czech hop that survived initial infection.
It was confirmed that a population of viroid was detectable in the leaves with a clearly dominant HpSVd-cucumber pale fruit viroid-like (HpSVd-cuc) sequence.
Besides HpSVd, also traces of truncated Citrus viroid (CVd IV) were detected in surviving biolistically infected hop samples.
In some samples, HpSVd was detectable by Northern blots, but not by RT PCR, suggesting some viroid sequence change(s) upon adaptation in hop.
An expression of several pathogenesis-associated marker genes were investigated.
It was found that experimentally induced viroid syndrome in Osvalds 72 led to gene deregulation and especially to strong depression of HlbHLH2 transcription factor participating in metabolome regulation in hop.
The disease was transferred using biolistic approach to Czech hop under isolated experimental conditions.
A complex of strong symptoms appeared on cultivar Osvalds 72 that was quite comparable to cultivar Celeia; low plant fitness strong stunting, leaf malformation, small (tiny) leaves and frequently visible leaf mosaic, which was not associated with virus infection. Hop stunt viroid (HpSVd) was analyzed in detail in the inoculated Czech hop that survived initial infection.
It was confirmed that a population of viroid was detectable in the leaves with a clearly dominant HpSVd-cucumber pale fruit viroid-like (HpSVd-cuc) sequence.
Besides HpSVd, also traces of truncated Citrus viroid (CVd IV) were detected in surviving biolistically infected hop samples.
In some samples, HpSVd was detectable by Northern blots, but not by RT PCR, suggesting some viroid sequence change(s) upon adaptation in hop.
An expression of several pathogenesis-associated marker genes were investigated.
It was found that experimentally induced viroid syndrome in Osvalds 72 led to gene deregulation and especially to strong depression of HlbHLH2 transcription factor participating in metabolome regulation in hop.
Publication
Authors
J. Matousek, S. Radisek, J. Jakse, G.S. Duraisamy, K. Uhlírová, L. Orctová, P. Svoboda, J. Patzak, J. Rausche
Keywords
viroid symptoms, biolistic, Pospiviroids, transcription factor, Humulus lupulus L., gene deregulation, Real Time quantitative RT-PCR
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