Articles
TECHNIQUE TO ASSESS GENE FUNCTION IN HOP (HUMULUS LUPULUS L.) – POWDERY MILDEW INTERACTIONS
Article number
1010_6
Pages
59 – 66
Language
English
Abstract
Improving resistance to powdery mildew (Podosphaera macularis ssp. humuli, Pm) is a major objective in hop breeding.
To achieve this, classical breeding can be assisted by marker-assisted breeding or by transgenic approaches.
For both these techniques, it would be of advantage to have DNA sequences available with confirmed function in the hop-powdery mildew interaction.
Here, we report on the adaptation of a transient transformation assay for the functional assessment of defence- or susceptibility-related genes in hop as already available for barley and wheat.
As a proof of concept, we performed transient-induced gene silencing (TIGS) of a putative hop powdery mildew susceptibility factor in hair cells, which resulted in a significantly reduced penetration success of the powdery mildew fungus.
To achieve this, classical breeding can be assisted by marker-assisted breeding or by transgenic approaches.
For both these techniques, it would be of advantage to have DNA sequences available with confirmed function in the hop-powdery mildew interaction.
Here, we report on the adaptation of a transient transformation assay for the functional assessment of defence- or susceptibility-related genes in hop as already available for barley and wheat.
As a proof of concept, we performed transient-induced gene silencing (TIGS) of a putative hop powdery mildew susceptibility factor in hair cells, which resulted in a significantly reduced penetration success of the powdery mildew fungus.
Publication
Authors
K. Oberhollenzer, E. Seigner, R. Eichmann, R. Hückelhoven
Keywords
hop breeding, resistance, defence-related genes, transient transformation assay, transient-induced gene silencing (TIGS), Podosphaera macularis
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