Articles
COMPARATIVE ANALYSIS OF WILD HOP BASED ON GENETIC, CHEMICAL AND MORPHOLOGICAL DATA
Article number
1010_7
Pages
67 – 76
Language
English
Abstract
Collections of wild hops are valuable resources of genetic variability useful for breeding of new cultivars with demanded properties.
For such purpose its necessary to do their characterization and classification.
Aim of our study was making a complex and comparative analysis by utilizing of genetic, chemical and morphological data.
Information about genetic variability was obtained by analysis of 20 SSR loci.
Chemo-classification was based on total compound of alpha and beta bitter acids, essential oils and 9 chemical substances.
It was used 13 different morphological parameters.
Thirty eight genotypes were analysed as per stringent criterion for full dataset of all type of markers.
Non-Metric Multidimensional Scaling (NMMDS) method was applied for statistical analysis.
Objective comparison was ensured by distance matrix based on Euclidean distances for all kind of markers.
The quality of fitting the proper model was determined by stress and alienation parameters with combination of Shepard diagram.
Chemo-classification has the best distinctive ability, but the highest information content was obtained by microsatellite markers.
Contrary, the least distinction was obtained from morphological datas.
Genetic analysis divided hops to the three isolated groups American, European and Caucasian.
Chemical profile mixed European and Caucasian types into one group.
Morphological characterization was able to distinguish between American and Caucasian group, however, many of European hops were overlapped with other groups.
Three dimensional models exposed excellent biological interpretation, because first two dimensions separated genetic isolated group of hops and third dimension distinguished two American varieties lupuloides and neomexicanus.
For such purpose its necessary to do their characterization and classification.
Aim of our study was making a complex and comparative analysis by utilizing of genetic, chemical and morphological data.
Information about genetic variability was obtained by analysis of 20 SSR loci.
Chemo-classification was based on total compound of alpha and beta bitter acids, essential oils and 9 chemical substances.
It was used 13 different morphological parameters.
Thirty eight genotypes were analysed as per stringent criterion for full dataset of all type of markers.
Non-Metric Multidimensional Scaling (NMMDS) method was applied for statistical analysis.
Objective comparison was ensured by distance matrix based on Euclidean distances for all kind of markers.
The quality of fitting the proper model was determined by stress and alienation parameters with combination of Shepard diagram.
Chemo-classification has the best distinctive ability, but the highest information content was obtained by microsatellite markers.
Contrary, the least distinction was obtained from morphological datas.
Genetic analysis divided hops to the three isolated groups American, European and Caucasian.
Chemical profile mixed European and Caucasian types into one group.
Morphological characterization was able to distinguish between American and Caucasian group, however, many of European hops were overlapped with other groups.
Three dimensional models exposed excellent biological interpretation, because first two dimensions separated genetic isolated group of hops and third dimension distinguished two American varieties lupuloides and neomexicanus.
Publication
Authors
J. Vasek, P. Vejl, D. Cílová, D. Zeka , V. Nesvadba, K. Krofta, A. Henychová , Z. Poloncíková
Keywords
Humulus lupulus L., microsatellite, bitter acids, essential oils, morphology, NMMDS
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