Articles
ATGR1 AND ATDHAR1 OVER-EXPRESSION IN TRANSGENIC PLANTS ENHANCES RECOVERY OF CRYOPRESERVED SHOOT TIPS OF POTATO (SOLANUM TUBEROSUM)
Article number
1039_10
Pages
91 – 96
Language
English
Abstract
Plant cells undergo various stresses during cryopreservation procedures.
Accumulation of reactive oxygen species (ROS) in plants is generally increased under stress conditions.
Oxidative damage may subsequently affect recovery of cryopreserved samples.
In the present study, we demonstrate that over-expression of the Arabidopsis thaliana glutathione reductase gene (AtGR1) or the Arabidopsis thaliana ascorbate reductase gene (AtDHAR1) in transgenic potato Atlantic significantly enhances recovery of shoot tips following PVS2 dehydration and LN exposure, compared with recovery of the wild-type. AtGR1 and AtDHAR1 over-expression may allow accumulation of reduced ascorbic acid (AsA) and glutathione (GSH), two of the more important antioxidants, in the transgenic plants.
Therefore, improved shoot recovery may be attributed to scavenging ROS which was produced during cryopreservation procedures.
This suggests that oxidative injury can be a critical factor affecting shoot recovery after cryopreservation.
Accumulation of reactive oxygen species (ROS) in plants is generally increased under stress conditions.
Oxidative damage may subsequently affect recovery of cryopreserved samples.
In the present study, we demonstrate that over-expression of the Arabidopsis thaliana glutathione reductase gene (AtGR1) or the Arabidopsis thaliana ascorbate reductase gene (AtDHAR1) in transgenic potato Atlantic significantly enhances recovery of shoot tips following PVS2 dehydration and LN exposure, compared with recovery of the wild-type. AtGR1 and AtDHAR1 over-expression may allow accumulation of reduced ascorbic acid (AsA) and glutathione (GSH), two of the more important antioxidants, in the transgenic plants.
Therefore, improved shoot recovery may be attributed to scavenging ROS which was produced during cryopreservation procedures.
This suggests that oxidative injury can be a critical factor affecting shoot recovery after cryopreservation.
Authors
Biao Wang, Jing-Wei Li, Qiao-Chun Wang, Shi-Wen Wang, Li-Na Yin
Keywords
ascorbic acid, glutathione, oxidative injury, ROS, transgenic-derived germplasm
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