Articles
ANTIOXIDANTS AND ANTI-STRESS COMPOUNDS IMPROVE THE SURVIVAL OF CRYOPRESERVED ARABIDOPSIS SEEDLINGS
Article number
1039_5
Pages
57 – 62
Language
English
Abstract
Cryopreservation is a safe and cost-effective tool for the long-term storage of plant germplasm.
Successful cryopreservation depends on suitable cryoprotection protocol.
In Arabidopsis seedlings cryopreservation, the growth ability could be partly restored in 60-h seedlings, whereas 72-h seedlings died after cryopreservation.
In this study, glutathione (GSH), ascorbic acid (VC), lipoic acid (LA), melatonin (M), polyvinylpyrrolidone (PVP), abscisic acid (ABA), glycine betaine (GB), CaCl2, and KNO3 were added to Plant Vitrification Solution 2 (PVS2) to improve the survival ratio in 60-h and 72-h seedlings.
Among of them, four compounds (GSH, VC, ABA and GB) increased the survival ratio approximately two times in 60-h seedlings cryopreservation, and FDA viability staining analysis also indicated that adding these four compounds made 60-h seedlings keep higher viability than control.
Other exogenous compounds had neutral or negative impacts on 60-h seedlings regrowth.
Moreover, in 72-h seedlings cryopreservation, GSH, GB, ABA and VC increased the survival ratio from 0 to 14.3, 7, 4.5 and 3%, respectively.
These four compounds were usually considered as antioxidants or anti-stress compounds.
In general, vitrification-based cryopreservation improves survival of plant materials by increasing cell viscosity, also produces complex stresses especially oxidative stress.
Antioxidants and anti-stress compounds in cryopreservation protocol can significantly increase the regrowth of seedlings may be due to reducing or repairing the oxidative damage.
Successful cryopreservation depends on suitable cryoprotection protocol.
In Arabidopsis seedlings cryopreservation, the growth ability could be partly restored in 60-h seedlings, whereas 72-h seedlings died after cryopreservation.
In this study, glutathione (GSH), ascorbic acid (VC), lipoic acid (LA), melatonin (M), polyvinylpyrrolidone (PVP), abscisic acid (ABA), glycine betaine (GB), CaCl2, and KNO3 were added to Plant Vitrification Solution 2 (PVS2) to improve the survival ratio in 60-h and 72-h seedlings.
Among of them, four compounds (GSH, VC, ABA and GB) increased the survival ratio approximately two times in 60-h seedlings cryopreservation, and FDA viability staining analysis also indicated that adding these four compounds made 60-h seedlings keep higher viability than control.
Other exogenous compounds had neutral or negative impacts on 60-h seedlings regrowth.
Moreover, in 72-h seedlings cryopreservation, GSH, GB, ABA and VC increased the survival ratio from 0 to 14.3, 7, 4.5 and 3%, respectively.
These four compounds were usually considered as antioxidants or anti-stress compounds.
In general, vitrification-based cryopreservation improves survival of plant materials by increasing cell viscosity, also produces complex stresses especially oxidative stress.
Antioxidants and anti-stress compounds in cryopreservation protocol can significantly increase the regrowth of seedlings may be due to reducing or repairing the oxidative damage.
Authors
L. Ren, D. Zhang, X.H. Shen, B.M. Reed
Keywords
abscisic acid, ascorbic acid, cryopreservation, exogenous compounds, glutathione, glycine betaine, PVS2
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