Articles
STRATEGIES FOR IMPROVED EFFICIENCY WHEN IMPLEMENTING PLANT VITRIFICATION TECHNIQUES
Article number
1039_9
Pages
85 – 89
Language
English
Abstract
Cryopreservation technologies allow vegetatively propagated genetic resources to be preserved for extended lengths of time.
Once successful methods have been established, there is a significant time investment to cryopreserve gene bank collections.
Our research seeks to identify methods that could improve the efficiency of the cryopreservation process.
We estimate the labor and time required to cryopreserve shoot tips that are excised directly from field, greenhouse, or screenhouse conditions.
We also provide estimates of the time and labor required to introduce propagules into tissue culture, proliferate them, and then excise shoot tips for cryopreservation.
Additionally, we provide some estimates for the time it takes to perform a representative cryopreservation procedure.
Finally, we demonstrate that micrografting can be used to assess viability after liquid nitrogen exposure when shoot tips cannot be successfully regenerated when placed directly on a culture medium.
Although the micrografting procedure is more labor- and time-intensive, it can also be highly effective especially when recovery media are not well established.
Once successful methods have been established, there is a significant time investment to cryopreserve gene bank collections.
Our research seeks to identify methods that could improve the efficiency of the cryopreservation process.
We estimate the labor and time required to cryopreserve shoot tips that are excised directly from field, greenhouse, or screenhouse conditions.
We also provide estimates of the time and labor required to introduce propagules into tissue culture, proliferate them, and then excise shoot tips for cryopreservation.
Additionally, we provide some estimates for the time it takes to perform a representative cryopreservation procedure.
Finally, we demonstrate that micrografting can be used to assess viability after liquid nitrogen exposure when shoot tips cannot be successfully regenerated when placed directly on a culture medium.
Although the micrografting procedure is more labor- and time-intensive, it can also be highly effective especially when recovery media are not well established.
Authors
G.M. Volk, A. Shepherd, R.M. Bonnart
Keywords
cryopreservation, cost efficiency
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