DIAGNOSTICS OF ERWINIA AMYLOVORA (BURR.) WINSLOW ET AL. ISOLATES CAUSING FIRE BLIGHT IN POME FRUIT TREES IN BURSA

Fire blight caused by Erwinia amylovora (Burr.) Winslow et al., is the most serious and destructive bacterial disease of pome fruit trees.
In 2011, in Bursa, bacterial isolations were conducted from different hosts and cultivars belonging to Rosaceae family.
This study represents 311 plant samples from pear, apple and quince, which resulted in a total of 120 E. amylovora isolates that were identified and characterized on the basis of biochemical and pathological tests.
All isolates were Gram negative and produced levan-type colonies when cultured on SNA (saccharose nutrient agar) and MS (Miller and Scroth) media.
Additionally, orange-colony phenotypes were observed when isolates were cultured on MS medium.
Pathogenicity tests on immature pear slices resulted in observation of milky ooze after incubation.
Isolates were grown on King B’s agar resulting in non-fluorescent colonies.
Bacteria were negative for oxidase, growth at 36°C, indole production, and generation of H2S from cysteine; positive for catalase activity and gelatin hydrolysis.
Oxidative/fermentative tests resulted in oxidative growth.

XIII International Workshop on Fire Blight

N. Tunali, M. Mirik

fire blight, Erwinia amylovora, apple, pear, quince

https://doi.org/10.17660/ActaHortic.2014.1056.20