Articles
COMPARATIVE GENOMICS OF ERWINIA AMYLOVORA ISOLATES FROM MEXICO
Article number
1056_27
Pages
173 – 177
Language
English
Abstract
Isolates of Erwinia amylovora from Mexico were collected and tested for their resistance to antibiotics used in orchards.
Isolates with resistance to streptomycin were found, but no resistance to the commonly used antibiotics tetracycline and gentamicin was detected.
The genomes of three isolates were sequenced and compared to reference genomes.
The streptomycin resistance of two isolates was due to the common K43R mutation in rpsL. Analysis of the genomes revealed that the strains belong to CRISPR group I, which is common with strains from the Northeastern region of the USA and that each strain has a mutation in avrRpt2, which is present in strains that are able to overcome the resistance of Malus × robusta 5.
Isolates with resistance to streptomycin were found, but no resistance to the commonly used antibiotics tetracycline and gentamicin was detected.
The genomes of three isolates were sequenced and compared to reference genomes.
The streptomycin resistance of two isolates was due to the common K43R mutation in rpsL. Analysis of the genomes revealed that the strains belong to CRISPR group I, which is common with strains from the Northeastern region of the USA and that each strain has a mutation in avrRpt2, which is present in strains that are able to overcome the resistance of Malus × robusta 5.
Publication
Authors
T.H.M. Smits, V.M. Guerrero-Prieto, G. Hernandez-Escarcega, F. Rezzonico, J. Blom, A. Goesmann, B. Duffy, V.O. Stockwell
Keywords
fire blight, genome sequencing, streptomycin resistance, CRISPR
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